Cloning and Sequential Analysis of the Cytokine from Mouse

Chun Fang Wang; Xiu Yun Jiang; Hua Rui Qi; Jia Yi Qiu; Chun Feng Wang; Ai Dong Qian

doi:10.4028/www.scientific.net/AMR.1030-1032.2340

Paper Titles

A Silicon Resonant Micro-Cantilever Biosensor with Closed-Loop Self-Excitation System for Biomacromolecular Detection
p.2320

Application of Rapid Prototyping Technology on Development of Medical Equipment
p.2326

A Design of the Bone Conduction Ultrasonic Hearing Device
p.2330

Biphasic Enantioselective Partitioning of R,S-Omeprazole Enantiomers Using Chiral Extraction
p.2334

Cloning and Sequential Analysis of the Cytokine from Mouse
p.2340

Computer Operator Cervical Disease Prevention and Physical Exercise
p.2344

Effects of Using Fine Quarry Waste as Cement Replacement Material on the Compressive Strength of the Mixture of Interlocking Block
p.2348

A Novel Biosensor Based on LAPS for the Detection of IgG
p.2354

An Auditory Brain-Computer Interface Based on Dichotic Listening Paradigm
p.2360

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1030-1032Cloning and Sequential Analysis of the Cytokine...

Cloning and Sequential Analysis of the Cytokine from Mouse

Abstract:

To analyze the diversity of expression quantity and lay foundation for real time fluorescent quantitation PCR, spleen samples of mouse were collected and mRNA were abstracted, furthermore, the mRNA were reverse transcribed into cDNA by using kit. Six cytokine genes IL-6, IL-12a, IL-12b, IFN-γ, TNF-αand iNOS were cloned and sequenced. The results demonstrated that the homogeneities of the sequences between amplified genes and cytokine genes in genebank all reached above 99%.

You might also be interested in these eBooks

Materials, Transportation and Environmental Engineering II

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 1030-1032)

Pages:

2340-2343

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1030-1032.2340

Citation:

Cite this paper

Online since:

September 2014

Authors:

Chun Fang Wang, Xiu Yun Jiang, Hua Rui Qi, Jia Yi Qiu, Chun Feng Wang, Ai Dong Qian*

Keywords:

Cloning, Cytokine Gene, Mouse, Sequential Analysis

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] S. Akira, T. Hirano, T. Taga and T. Kishimoto: FASEB J. Vol. 4(1990), p.2860.

Google Scholar

[2] D. L. Barber, B. B. Andrade, C. McBerry, I. Sereti and A. Sher: J Immunol, Vol. 192(2014), p.676.

Google Scholar

[3] C. A. Coutinho, F. A. Marson, A. R. Marcelino, L. C. Bonadia, M. P. Carlin, A. F. Ribeiro, J. D. Ribeiro and C. S. Bertuzzo: Int J Mol Epidemiol Genet, Vol. 5(2014), p.87.

Google Scholar

[4] A. Vrba-Pech, M. Fol, M. Krawczyk, M. Kowalewicz-Kulbat and S. Kwiatkowska: Tuberculosis (Edinb), Vol. 94(2014), p.383.

DOI: 10.1016/j.tube.2014.04.003

Google Scholar

[5] W. Huang, Y. Qi, C. Ren, H. Wen, K. L. Franken, T. H. Ottenhoff and J. Shen: Tuberculosis (Edinb), Vol. 93(2013), p.612.

Google Scholar

[6] O. L. de, E. Peresi, M. A. Golim, M. Gatto, J. J. Araujo, C. E. da, J. A. Ayres, M. R. Fortes and S. A. Calvi: PLoS One, Vol. 9(2014), p. (2014).

Google Scholar

[7] A. Raja: Indian Journal of Medical Research, Vol. 120(2004), p.213.

Google Scholar

[8] J. Eun-Kyeong, P. Jeong-Kyu and M. D. Hazel: Curr Opin Infect Dis, Vol. 16 (2003), p.205.

Google Scholar

[9] S.H.E. Kaufmann: Ann Rheum, Vol. 61(2002), p.1154.

Google Scholar

[10] Y. Yuhas, E. Berent, R. Cohen and S. Ashkenazi: Antimicrobial Agents and Chemotherapy, Vol. 53(2009), p.1539.

Google Scholar

[11] J. MacMicking, Q.W. Xie and C. Nathan: Annu Rev Immunol, Vol. 15(1997), p.323.

Google Scholar