A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on ITS Sequences

Shao Xuan Zhang; Guang Zhu Lin; Bo Chuan Wang; Yan Shi

doi:10.4028/www.scientific.net/AMR.1033-1034.179

Paper Titles

Research on Influencing Factor of Performance Ability of Emulsion Explosive
p.160

Relationship between the Property of Cloud Point and CO₂ Absorption and Desorption of Amines
p.164

By Using FE-Fe₂O₃ Galvanic Pair in Sulphate Solution for Obtaining of a Chemical Source of a Current
p.170

A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on Chloroplast trnL-F Sequences
p.175

A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on ITS Sequences
p.179

Alkaline Delignification of Banana Peel for Producing Pulp
p.183

Analysis on Change of Nutritional Ingredients in Ensiling Process of Pineapple Leaf Residue
p.187

Biosynthesis of γ-Decalactone with Lipase-Catalyzed Resolution from Castor Oil by Saccharomyces cerevisiae
p.193

Chemical Characterization of the Essential Oils of Eucalyptus.grandis×Eucalyptus.urophylla Hybrids and Six Pure Eucalyptus species Grown in Guangxi (China)
p.200

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034A New Method to Identify Pulsatilla chinesis(Bge)...

A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on ITS Sequences

Abstract:

To establish a reliable identification method of Pulsatilla chinesis (Bge) Regel., from its counterfeits, Potentilla chinesis Ser., we extracted the genome DNA from the leaves of these two plants collected in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and compared. Based on ITS sequences, we used primer PREMIER 5.0 to search the restriction sites of the two ITS sequences and found BtgI can be used to identify them.

You might also be interested in these eBooks

Advances in Chemical Engineering and Advanced Materials IV

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

179-182

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.179

Citation:

Cite this paper

Online since:

October 2014

Authors:

Shao Xuan Zhang, Guang Zhu Lin*, Bo Chuan Wang, Yan Shi

Keywords:

ITS, Potentilla chinesis Ser., Pulsatilla Chinesis (Bge.) Regel, Sequencing: Identification

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] Chinese Pharmacopoeia Commisson in: Pharmacopoeia of The People's Republic of China, Beijing: People's Medical Publishing House, China(2005), pp.231-232.

Google Scholar

[2] Chinese Pharmacopoeia Commisson in: Pharmacopoeia of The People's Republic of China, Beijing: People's Medical Publishing House, China(2005), pp.148-149.

Google Scholar

[3] New Medical College of Jiangsu in : A Dictionary of Traditional Chinese Medicine (1st ed. ), Shanghai Sience and Technology Press, China(1985), in pp.704-706. (in Chinese).

Google Scholar

[4] D.L. Zhang, X. Fu, Y.Y. Yang: Chinese Traditional and Herbal Drugs. Vol. 31(2000), pp.554-556 (in Chinese).

Google Scholar

[5] S.Q. Wang: LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH. Vol. 12(2001), pp.428-429 (in Chinese).

Google Scholar

[6] F. Takaiwa, K. Oono, M. Sugiura: Plant Molecular Biology. Vol. 4(1985), pp.355-364.

Google Scholar

[7] S.X. Zhang, F. Liu, Y.H. Ling, B.C.　Wang, D.J. Sun, G.Z. Lin:. Advanced Material Research Vol. 749(2013). pp.246-249.

Google Scholar