Biosynthesis of γ-Decalactone with Lipase-Catalyzed Resolution from Castor Oil by Saccharomyces cerevisiae

Abstract:

Castor oil is the main feedstock to produce γ-decalactone among many materials. In this paper, an approach to efficiently improve the production of γ-decalactone by adding porcine pancreatic lipase was described. Saccharomyces cerevisiae strain with the highest production efficiency of γ-decalactone was firstly selected from ten yeasts. Then, the culture conditions were optimized. During the fermentation at an optimal temperature of 35°C, adding 1.0g L^-1 porcine pancreatic lipase and 4g L^-1 Tween 80 promoted the capacity of γ-decalactone production. With supplement of 1% castor oil at the 24 h, γ-decalactone production reached as high as 3.10g L^-1 after a fermentation of 64 h. The production increased about 20-fold compared with the fermentation without adding porcine pancreatic lipase.

• Periodical: Advanced Materials Research (Volumes 1033-1034)
• Pages: 193-199
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.193
• Online since: October 2014
• Authors: Shun Li Cao, Qian Qian Li*, Shao Feng Rong, Bao Guo Cai, Shi Min Guan, Shu Lin Yang
• Keywords: Castor Oil, Lipase-Catalyze, Porcine Pancreatic Lipase, Saccharomyces cerevisiae, γ-Decalactone

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