A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on Chloroplast trnL-F Sequences

Shao Xuan Zhang; Guang Zhu Lin; Bo Chuan Wang; Yan Shi

doi:10.4028/www.scientific.net/AMR.1033-1034.175

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034A New Method to Identify Pulsatilla chinesis(Bge)...

A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on Chloroplast trnL-F Sequences

Abstract:

To establish a reliable identification method of Pulsatilla chinesis (Bge) Regel., from its counterfeits, Potentilla chinesis Ser., we extracted the genome DNA from the leaves of these two plants collected in Jilin Province, amplified the chloroplast trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and compared. Based on trnL-F sequences, we used primer PREMIER 5.0 to search the restriction sites of the two trnL-F sequences and found RsaI can be used to identify them.

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Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

175-178

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.175

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Online since:

October 2014

Authors:

Shao Xuan Zhang*, Guang Zhu Lin, Bo Chuan Wang, Yan Shi

Keywords:

Chloropalst, Potentilla chinesis Ser., Pulsatilla Chinesis (Bge.) Regel, Sequencing: Identification, trnL-F

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