Effect of GDM on the Expression of MT1-MMP and u-PA in Glioma Cells

Xue Mei Han; Li Bo Wang; Ni Ni Li; Song Yan Liu

doi:10.4028/www.scientific.net/AMR.1033-1034.220

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034Effect of GDM on the Expression of MT1-MMP and...

Effect of GDM on the Expression of MT1-MMP and u-PA in Glioma Cells

Abstract:

To examine the effect of GDM on the expression of MT1-MMP and u-PA genes in glioma cells. Glioma cell lines U251 and U87 were cultured in DMEM medium supplemented with 10% fetal bovine serum. RT-PCR was used to identify gene expression level. The level of u-PA mRNA was up-regulated significantly in the HGF group compared with the normal control group (P<0.05). The expression of MT1-MMP and u-PA was significantly lower in the GDM group than in the normal control and HGF groups (P<0.05). The expression of u-PA in the HGF+GDM group was down-regulated significantly compared with the normal control and HGF groups (P<0.05).GDM can inhibit expression of both MT1-MMP and u-PA in glioma cells.

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Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

220-223

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.220

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Online since:

October 2014

Authors:

Xue Mei Han*, Li Bo Wang, Ni Ni Li, Song Yan Liu

Keywords:

Geldanamycin, Glioma, Hepatocyte Growth Factor, Membrane Type 1 Matrix Metalloproteinase, Urokinase-Type Plasminogen Activator

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