Effect of Geldanamycin on the Glioma Cell Invasion Induced by Hepatocyte Growth Factor

Abstract:

To investigate the effect of geldanamycin (GDM) on the invasion ability of glioma cell induced by hepatocyte growth factor (HGF). Malignant glioma cell line U251-MG and U87-MG were cultured’and the capability of cell invasion was detected using a Transwell culture system. HGF significantly promoted the invasion ability of both U251-MG and U87-MG cells as compared with the normal control (NC) (P < 0.05). Forty eight hours after GDM treatment, the invasive growth of glioma cells was significantly decreased as compared with either NC or HGF group (P < 0.05). When cells were exposed to GDM plus HGF for 48 h, the cell invasion capability was greatly reduced as compared with either NC or HGF group (P < 0.05). The number of invaded cells in GDM plus HGF group was similar to that of GDM group.GDM can inhibit the invasion ability of glioma cells induced by HGF.

• Periodical: Advanced Materials Research (Volumes 1033-1034)
• Pages: 224-228
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.224
• Online since: October 2014
• Authors: Yan Xia Sun, Guang Yu Zhou, Li Li, Xue Mei Han*
• Keywords: Geldanamycin, Glioma, Hepatocyte Growth Factor, Invasion Ability

References

[1] Tsou HK, Chen HT, Hung YH, et al. HGF and c-Met interaction promotes migration in humanc [J]. PLoS One. 2013, 8(1): e53974.

DOI: 10.1371/journal.pone.0053974

[2] Groblewska M, Siewko M, Mroczko B, et al. The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer [J]. Folia Histochem Cytobiol. 2012, 50(1): 12-19.

DOI: 10.5603/fhc.2012.0002

[3] Sun C, Wang Q, Zhou H, et al. Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo [J]. Neurosci Bull. 2013, 29(1): 83-93.

DOI: 10.1007/s12264-012-1296-5

[4] Mou L, Kang Y, Zhou Y, et al. Neurokinin-1 receptor directly mediates glioma cell migration by up-regulation of matrix metalloproteinase-2 (MMP-2) and membrane type 1-matrix metalloproteinase (MT1-MMP) [J]. J Biol Chem. 2013, 288(1): 306-318.

DOI: 10.1074/jbc.m112.389783

[5] Kesanakurti D, Chetty C, Rajasekhar Maddirela D, et al. Functional cooperativity by direct interaction between PAK4 and MMP-2 in the regulation of anoikis resistance, migration and invasion in glioma [J]. Cell Death Dis. 2012, 3: e445.

DOI: 10.1038/cddis.2012.182

[6] SunYN, YangBF, LiuM, et al. The experiment on MMP-2andMMP-9gene simultaneouslysilencing inhibited the growth of the laryngeal cancer invasion [J]. Chinese Medical Journal 2008, 88(1): 36-39.

[7] Jung JS, Jung K, Kim DH, et al. Selective inhibition of MMP-9 gene expression by mangiferin in PMA-stimulated human astroglioma cells: involvement of PI3K/Akt and MAPK signaling pathways [J]. Pharmacol Res. 2012, 66(1): 95-103.

DOI: 10.1016/j.phrs.2012.02.013

[8] Yamamoto Y, Osanai T, Nishizaki F, et al. Matrix metalloprotein-9 activation under cell-to-cell interaction between endothelial cells and monocytes: possible role of hypoxia and tumor necrosis factor-alpha [J]. Heart Vessels. 2012, 27(6): 624-633.

DOI: 10.1007/s00380-011-0214-5

[9] WANG CH, Luo YN, Li YQ, Effect of GDM on the expression of MT1-MMP and inglioma cells [J]. Chinese Journal of Gerontology, 2008, 28(1): 11-13.