Enhanced Membrane Permeability of Saccharomyces cerevisiae Induced by Dielectric Barrier Discharge Plasma at Atmospheric Pressure

Xiao Yu Dong

doi:10.4028/www.scientific.net/AMR.1033-1034.229

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Enhanced Membrane Permeability of Saccharomyces cerevisiae Induced by Dielectric Barrier Discharge Plasma at Atmospheric Pressure

Abstract:

In this study, we investigated the effect of DBD plasma at atmospheric pressure on membrane permeability, potential membrane and cytoplasmic free calcium concentration in Saccharomyces cerevisiae. S. cerevisiae cells were treated with DBD plasma for 0, 1, 2, 3, 4 and 5 min and then subjected to various analyses before the commencement of fermentation or at the middle (9 h) and end (21 h) stages of fermentation. The results show that compared to non-treated cells, cells treated with plasma for 2-5 min show significant increase in membrane potential, while cells treated for 2-4 min show significant increase in membrane permeability prior to fermentation. Plasma-treated cells also show increased cytoplasmic free Ca²⁺ that depends on plasma treatment time, with the highest increase, 35%, observed for 5-min treatment time. However, at the middle (9 h) and end (21 h) stages of fermentation, these changes become insignificant compared to the control.

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Periodical:

Advanced Materials Research (Volumes 1033-1034)

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229-235

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https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.229

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Online since:

October 2014

Authors:

Xiao Yu Dong*

Keywords:

Cell Membrane Permeability, Cell Membrane Potential, Dielectric Barrier Discharge, Intracellular Calcium Concentration, Saccharomyces cerevisiae

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