Research of the Unknown Cause of Death of Caesio diagramma

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To find out the etiology and prevention methods of the death of Caesio diagramma. Isolate the pathogen from the fester surface, liver, kidney , intestinal and other organizations of the diseased Caesio diagramma; After the purification of the predominant strains, the phylogenetic analysis of genetic relationships was done by determining 16s rDNA gene and Homology comparison was done with the BLAST sequence detected and registered in GenBank sequence ; In the end, phylogenetic trees were constructed and the drug sensitivity experiment and pathological observation were taken. Aeromonas hydrophila is the cause of the death of Caesio diagramma, it is highly sensitive to chloramphenicol, florfenicol, cefotaxime, gentamicin, amikacin; The pathological results indicate that the wax buildups causes increasing energe consumption of Caesio diagramma,which decreased the resistance of Caesio diagramma and even leads to secondary bacterial infection. Pathogen infected the liver and spleen tissues which causes the disease and death of Caesio diagramma. Aeromonas hydrophila causes the death of Caesio diagramma, and the results have an important guiding significance for the healthy breeding of Caesio diagramma and other ornamental fish

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373-377

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October 2014

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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