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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1051In Vitro Cytotoxic Studies of Ultrafine...

In Vitro Cytotoxic Studies of Ultrafine TiO₂/Graphene Oxide Sheet Nanocomposites in Human Alveolar Epithelial Cells

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Abstract:

The novel TiO₂/graphene sheet (TiO₂/GSs) nanocomposites were synthesized using graphene oxide sheets and TiCl₃. All target composites were characterized by transmission electron microscopy (TEM), X-ray electron diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectra (EDX), and the surface areas were tested with the Brunauer-Emmett-Teller (BET) method. We studied the cytotoxicity of TiO₂/GSs nanoparticles on A549 cells by examining the influence of TiO₂/GSs on cell morphology and viability by methyl thiazolyl tetrazolium (MTT) assay. We also determined membrane integrity and apoptosis of A549 cells after different doses of TiO₂/GSs exposure by LDH assay and flow cytometry. Assay of A549 cell viability showed regular reductions with a time-and dose-dependent tendency after exposure to pure TiO₂, TiO₂/4.2wt% GSs and TiO₂/6wt% GSs for 24 hrs or 48 hrs. The LDH released and cellular apoptosis also had a dose-dependent effect, which was associated with the surface area of TiO₂/4.2wt% GSs. Our results provide essential knowledge of the acceptable biocompatibility of TiO₂/GSs nanocomposites, and only when cells were exposed at a high concentration (≥50 μg/mL), and for a prolonged period of time did TiO₂/GSs nanoparticles exhibit minimal cytotoxicity against A549 cells.

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Periodical:

Advanced Materials Research (Volume 1051)

Pages:

353-362

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1051.353

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Online since:

October 2014

Authors:

Jing Gao, Xue Shun Yuan, Yu Wang, Lin Cao, Peng Yu Dong, Bao Cheng Cao*

Keywords:

A549 Cells, Apoptosis, Cell Viability, LDH, Nanocomposites, TiO₂/GSs

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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