Isolation, Identification, and Characterization of an Organophosphorous Pesticide Degrading Bacterium, Enterobacter ludwigii M2

Abstract:

Strain M2 which can degrade organophosphorus pesticide residues was isolated from the soil. It could tolerate concentrations up to 500mg/L of methyl parathion, 500mg/L of chlorpyrifos and 1000mg/L of dimethoate in mineral salt medium containing 0.5% sucrose. In the soil test, strain M2 could degrade 93% of methyl parathion in the soil within 32 days, 71% of chlorpyrifos within 54 days. The degradation rate of strain M2 is much greater than that of natural microorganisms, soil adsorption and the capacity of natural degradation. Strain M2 was identified as a pesticide-degrading strain of Enterobacter ludwigii according to phenotype, biochemical properties and 16S rDNA sequence analysis. The type strain was M2 deposited in China General Microbiological Culture Collection Center on June 8, 2009 and the collection number is CGMCC NO.3092.

• Periodical: Advanced Materials Research (Volume 1051)
• Pages: 398-403
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1051.398
• Online since: October 2014
• Authors: Ying Zhao, Ping Zhao, Ya Wang, Weng Jing Qi
• Keywords: Enterobacter ludwigii, Isolation, Microbial Degradation, Organophosphorous Pesticide

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