Optimization for the Process of Chlorophyll a Extraction by Orthogonal Method

Jun Sen Wu; Chen Chen Liu; Bing Li; Xiu Zhi Wan

doi:10.4028/www.scientific.net/AMR.1051.423

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1051Optimization for the Process of Chlorophyll a...

Optimization for the Process of Chlorophyll a Extraction by Orthogonal Method

Abstract:

The 90% acetone as extraction agent used in order to obtain the ultrasonic assisted extraction conditions of chlorophyll a. The ultrasonic power, ultrasonic time, centrifugation time and rotational speed as the four study factors and the chlorophyll a concentration as the target, here, the L9(34) orthogonal table of orthogonal experiment design are used in order to determine the optimum extraction contractions, basing on the single factor experiment. The experimental results shows the algae containing water can get better extraction effect, after ultrasonic 30 minutes in 225W and 6000r/min speed centrifugal 9 minutes and then mixing, repeat ultrasound centrifugation steps.

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Periodical:

Advanced Materials Research (Volume 1051)

Pages:

423-427

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1051.423

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Online since:

October 2014

Authors:

Jun Sen Wu*, Chen Chen Liu, Bing Li, Xiu Zhi Wan

Keywords:

Acetone, Chlorophyll-a, Extraction, Orthogonal Experiment, Ultrasonic

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