Preparation of Nanometer Chitosan - RNA Composite and the Role in Induced Type I Interferon

Abstract:

This paper used double step compounding chitosan nanoparticles as carrier, took the virus's outer membrane NS1 shRNA delivery to cells,and simultaneous detection and characterization of chitosan-RNA Nanocomposites.The experiment results show that properties of chitosan-RNA nanocomposite is stable, using chitosan-RNA Nanocomposites as carrier carry shRNA transfecting B16-Blue INF-alpha/beta cells, shRNA can’t degradation by nuclear enzyme, improve cell absorbing and shRNA intracellular release.Experiments further confirmed that the chitosan - RNA Nanocomposites into the cell, NS1 shRNA is identified by RIG-I gene, induces gene expression of interferon, produces interferonItype, offers a new way for the application of new type of flu vaccine.

• Periodical: Advanced Materials Research (Volume 1051)
• Pages: 43-47
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1051.43
• Online since: October 2014
• Authors: Li Hui Zhao, He Nan Zhao, De Ming Han*
• Keywords: B16-Blue INF-α/βCell, Carrier, Chitosan - RNA Nanocomposites, Type I Interferon

References

[1] Li BJ, tang Q, Cheng D. Using siRNA in ProPhylactic and therapeutic Regimens against SARS coronaVirus in Rhesus macaque, NatMed. 11(2005)944-951.

DOI: 10.1038/nm1280

[2] Hommel JD, Sears RM, Georgescu D. Local gene lockdown in the brain using viral-mediated RNA inierferenee, NatMed. 9(2003)1539-1344.

[3] Singer O, Marr RA, Rockenstein E. Targeting BACEI with siRNAs ameliorates Alzheimer disease neuropathology in a transgenic model, Nat Neurosci. 8(2005)1343-1349.

DOI: 10.1038/nn1531

[4] Howard KA, Rahbek UL, Liu X, et al. RNA Interference in Vitro and in Vivo Using a Novel Chitosan/siRNA Nanoparticle System, Mol Ther. 14(2006) 476-484.

DOI: 10.1016/j.ymthe.2006.04.010

[5] Liu X, Howard KA, Dong M. Thein fluence of Polymeric ProPerties on Chitosan/siRNA nanoparticle formulation and gene silencing, Biomaterials. 28(2007)1280-1288.

DOI: 10.1016/j.biomaterials.2006.11.004

[6] Dykman L, Khlebtsov, N. Gold Nanoparticles in Biomedical Applications: Recent Advances and Perspectives, Chem. Soc. Rev. 27(2012) 2256-2282.

DOI: 10.1039/c1cs15166e

[7] Slocik, J. M., Govorov, A. O., Naik, R. R. Plasmonic Circular Dichroism of Peptide-Functionalized Gold Nanoparticles, Nano Lett. 11(2011) 701-705.

DOI: 10.1021/nl1038242

[8] Takaoka A. et al. DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response, Nature. 448(2007) 501-505.

DOI: 10.1038/nature06013

[9] Ishii KJ. et al. TANK-binding kinase-1 delineates innate and adaptive immune responses to DNA vaccines, Nature. 451(2008) 725-729.

DOI: 10.1038/nature06537

[10] Cheng G. et al. Double-stranded DNA and double-stranded RNA induce a common antiviral signaling pathway in human cells, Proc Natl Acad Sci U S A. 104(2007) 9035-9040.

DOI: 10.1073/pnas.0703285104

[11] Yang P. et al. The cytosolic nucleic acid sensor LRRFIP1 mediates the production of type I interferon via a beta-catenindependent pathway, Nat Immunol. 11(2010) 487-494.

DOI: 10.1038/ni.1876

[12] Jones Jw. et al. Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis, PNAS. 107(2010) 9771-9776.

[13] Vivier, E., Raulet, D. H., Moretta, A., Caligiuri, M. A., Zitvogel, L., Lanier,L. L., Yokoyama, W. M., Ugolini, S. Innate or adaptive immunity?The example of natural killer cells. Science , 331(2011)44-49.

DOI: 10.1126/science.1198687

[14] Paust, S., Senman, B., von Andrian, U. H. Adaptive immune responses mediated by natural killer cells, Immunol. Rev. 235(2010)286-296.

DOI: 10.1111/j.0105-2896.2010.00906.x