Synthesis and Characterization of a Cascaded Chromogenic Enzyme Substrate

Hai Xin Ding; Zhi Zhong Ruan; Qiang Xiao; Xiao Jun Wang

doi:10.4028/www.scientific.net/AMR.1094.37

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Synthesis and Characterization of a Cascaded Chromogenic Enzyme Substrate
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1094Synthesis and Characterization of a Cascaded...

Synthesis and Characterization of a Cascaded Chromogenic Enzyme Substrate

Abstract:

A novel galactoside derivative (1) incorporating p-nitroaniline (p-NA) as chromophore has been synthesized and used as a cascaded chromogenic enzyme substrate for the detection of β-D-galactosidase, which was characterized using UV, ¹H NMR, ¹³C NMR and MS spectra. A plausible mechanism for the release of p-NPA from the enzyme substrate 1 was proposed.

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Periodical:

Advanced Materials Research (Volume 1094)

Pages:

37-40

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1094.37

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Online since:

March 2015

Authors:

Hai Xin Ding*, Zhi Zhong Ruan, Qiang Xiao, Xiao Jun Wang

Keywords:

Cascaded Response, Chromogenic Substrate, Detection, E. coli, P- Nitroaniline

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