Retarding Bioactivity Effect of Ciprofloxacin Incorporated in Polyvinyl Alcohol/Bioglass Scaffold as Bone Graft

Abstract:

The biomaterials are used for many biomedical applications. The main objective of the present work was to investigate the potential role of Bioglass (Melting)- Polyvinyl alcohol (BG (M)-PVA) and Bioglass (Melting)-Polyvinyl alcohol-20%Ciprofloxacin (BG (M)-PVA-20Cip) in regenerative bone capacity. These composites were implanted in the femoral condyles of Wistar rats and compared to that of ovariectomised groups. Our results noted, after the different period of implantation (15, 30, 60 and 90 days), that the Alkaline phosphatase (ALP) and Acid phosphatase (ACP) activities showed an excellent osteoinductive property of BG (M)-PVA, that this phenomena decreased with the presence of ciprofloxacin. Physico-chemical techniques (ICP-OES and SEM) were engaged to highlight the influence of antibiotic on the structure, porosity and bioactivity of a porous Glass-PVA before and after implantation. The results obtained by ICP-OES showed a rapid reduction in silicon (Si) and sodium (Na), and noted an accelerator increase in calcium (Ca) and phosphorus (P) ion concentrations in BG (M)-PVA that the BG (M)-PVA-20Cip. This result is confirmed by SEM. We can conclude that the loading of ciprofloxacin in BG (M)-PVA is characterized by a retard effect of formation of apatitic phase.

• Periodical: Advanced Materials Research (Volume 1104)
• Pages: 163-167
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1104.163
• Online since: May 2015
• Authors: Salha Boulila, Hassane Oudadesse*, Bertrand Lefeuvre, Khansa Chaabouni, Fatima Makni-Ayedi, Pascal Pellen, Hafedh El Feki, Allal Barroug, Abdelfattah El Feki
• Keywords: Bioactivity, Bioglass, Ciprofloxacin, Polyvinyl Alcohol, Retard

References

[1] S. Boulila, K. Mnafgui, , H. Oudadesse , H. El Feki, A. El Feki, Comparison of three types of physical aspects of a carbonated hydroxyapatite biomaterial: Study implantaion in vivo in rats of Wistar, strain and physiological & physicochemical explorations, Journal of Advances in Chemistry. 8 (2014).

DOI: 10.24297/jac.v8i2.4042

[2] W. Pon-On, N. Charoenphandhu, J. Teerapornpuntakit, J. Thongbunchoo, N. Krishnamra, I.M. Tang, Mechanical properties, biological activity and protein controlled release by poly(vinyl alcohol)–bioglass/chitosan–collagen composite scaffolds: A bone tissue engineering applications, Materials Science and Engineering C. 38 (2014).

DOI: 10.1016/j.msec.2014.01.040

[3] H.H. Pham,  P. Luo, F. Génin, A.K. Dash, Synthesis and characterization of HA-ciprofloxacin delivery systems by precipitation and spray drying technique, AAPS Pharm Sci Tech. 3 (2002) 1–9.

DOI: 10.1208/pt030101

[4] M. Sundar Raj, V. Arkin, H. Adalarasu, M. Jagannath, Nanocomposites Based on Polymer and Hydroxyapatite for Drug Delivery Application, Indian Journal of Science and Technology. 6 (2013).

[5] V. Mouriño, J.P. Cattalini, J.A. Roether, P. Dubey, I. Roy, A.R. Boccaccini, Composite polymer-bioceramic scaffolds with drug delivery capability for bone tissue engineering, Expert Opin. Drug Deliv. 10 (2013) 1353 – 1365.

DOI: 10.1517/17425247.2013.808183

[6] E. Dietrich, H. Oudadesse, A. Lucas-Girot, M. Mami, In vitro bioactivity of melt-derived glass 46S6 doped with magnesium, J Biomed Mater Res A. 88 (2009) 1087-1096.

DOI: 10.1002/jbm.a.31901

[7] M. Mabrouk, M. Mostafa, H. Oudadesse, A.M. Gaafar, M.I. El-Gohary, Fabrication, Characterization and Drug Release of Ciprofloxacin Loaded Porous Polyvinyl Alcohol/Bioactive Glass Scaffold for Controlled Drug Delivery, Bioceramics Development and Applications. (2014).

DOI: 10.4172/2090-5025.s1-009

[8] K. Jung, M. Lein, Bone turnover markers in serum and urine as diagnostic, prognostic and monitoring biomarkers of bone metastasis, Biochimica et Biophysica Acta 1846, (2014) 425–438.

DOI: 10.1016/j.bbcan.2014.09.001

[9] C. Gao, Q. Gao, Y. Li, M.N. Rahaman, A. Teramoto, K. Abe, Preparation and in vitro characterization of electrospun PVA scaffolds coated with bioactive glass for bone regeneration, Journal of Biomedical Materials Research Part A. 100A(5) (2012).

DOI: 10.1002/jbm.a.34072

[10] A.M. Halawa, Effect of Ciprofloxacin on the Articular Cartilage and Epiphyseal Growth Plate Cartilage in the Growing Albino Rats and the Possible Protective Role of Vitamin E (α –Tocopherol): A Histological and Morphometric Study, Egypt. J. Histol. 33 (2010).

DOI: 10.21276/aanat.2015.1.1.4

[11] S. Jebahi, H. Oudadesse, H. El Feki, T. Rebai, H. Keskes, P. Pellen, A. El Feki Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats, J Appl Biomed. 10 (2012) 195–209.

DOI: 10.2478/v10136-012-0009-8

[12] C. Castro, E. Sanchez, A. Delgado, I. Soriano, P. Nunez, A. Perera, C. Evora, Ciprofloxacin implants for bone infection. In vitro–in vivo characterization, J. of Controlled Release. 9 (2003) 3341– 354.

DOI: 10.1016/j.jconrel.2003.09.004

[13] J.R. Jones, Review of bioactive glass: From Hench to hybrids, Acta Biomaterialia. 9 (2013) 4457–4486.

DOI: 10.1016/j.actbio.2012.08.023

[14] L.L. Hench, J.M. Polak, Third-generation biomedical materials, Science. 295 (2002) 1014–7.

[15] M. Grunewald, I. Avraham, Y. Dor, E. Bachar-Lustig, A. Itin, S. Yung, VEGF induced adult neovascularization: recruitment, retention, and role of accessory cells, Cell. 124 (2006) 175–89.

DOI: 10.1016/j.cell.2005.10.036