Wax-Impregnated Cotton Fabrics as Cell Culture Platform

Abstract:

Microwell plates are widely used in various cell-based assay and drug screening. Usually these plates are made from non-biodegradable materials such as polystyrene or polyethylene. In this report, we propose the use of wax-impregnated cotton fabrics as an alternative microwell plate that is easy to fabricate, simple to use, sustainable and environmentally friendlier. Several researchers recently used soft lithography and photolithography technique to fabricate various cell culture platforms. Our proposed method consists instead of simple dipping and drying process. The platform is made of a series of fabrics being stacked and held together with various wax formulations. With the exception of the base layer, each fabric layer has a circular hole opening with increasing diameter towards the top layer; thereby forming a well where cells are cultured at its flat bottom. We characterized the chemical and physical properties of the platform surface which affect cells attachment and proliferation. These properties include the surface chemistry, hydrophobicity and roughness. We cultured human skin fibroblast (HSF 1184 Cell Line) on the platform as our preliminary proof of concept. We observed proliferation of the cells after 24 hours. The result indicates the potential use of the platform for future cell-based assay applications.

• Periodical: Advanced Materials Research (Volume 1112)
• Pages: 441-444
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1112.441
• Online since: July 2015
• Authors: Norsamsiah Muhamad Wahab, Syazwani Abdul Jamil, Dwi Gustiono Riban, Fadzilah Adibah Abdul Majid, Mohammed Rafiq Abdul Kadir, Dedy Hermawan Bagus Wicaksono*
• Keywords: Cell Culture, Cell-Based Assay, Cotton Fabrics, Wax

References

[1] H. -C. Moeller, M. K. Mian, S. Shrivastava, B. G. Chung, and A. Khademhosseini, A microwell array system for stem cell culture, Biomaterials, . 9(6), (2008) 752-763.

DOI: 10.1016/j.biomaterials.2007.10.030

[2] T. G. van Kooten, H. T. Spijker, and H. J. Busscher, Plasma-treated polystyrene surfaces: model surfaces for studying cell–biomaterial interactions, Biomaterials. 25 (2004) 1735-1747.

DOI: 10.1016/j.biomaterials.2003.08.071

[3] Z. Shourgashti, M. T. Khorasani, and S. M. E. Khosroshahi, Plasma-induced grafting of polydimethylsiloxane onto polyurethane surface: Characterization and in vitro assay, "Radiation Physics and Chemistry. 79 (2010) 947-952.

DOI: 10.1016/j.radphyschem.2010.04.007

[4] H. -H. Shuai, C. -Y. Yang, H. I. C. Harn, R. L. York, T. -C. Liao, W. -S. Chen, et al., Using surfaces to modulate the morphology and structure of attached cells - a case of cancer cells on chitosan membranes, Chemical Science. 4 (2013) 3058-3067.

DOI: 10.1039/c3sc50533b

[5] M. Ni, W. H. Tong, D. Choudhury, N. A. A. Rahim, C. Iliescu, and H. Yu, Cell culture on MEMS platforms: A review, International journal of molecular sciences. 10 (2009) 5411-5441.

DOI: 10.3390/ijms10125411

[6] S. Selimovic, F. Piraino, H. Bae, M. Rasponi, A. Redaelli, and A. Khademhosseini, Microfabricated polyester conical microwells for cell culture applications, Lab on a Chip. 11 (2011) 2325-2332.

DOI: 10.1039/c1lc20213h

[7] Y. Guan and W. Kisaalita, Cell adhesion and locomotion on microwell-structured glass substrates, Colloids and Surfaces B: Biointerfaces. 84 (2011) 35-43.

DOI: 10.1016/j.colsurfb.2010.12.007

[8] G. Altankov, F. Grinnell, and T. Groth, Studies on the biocompatibility of materials: Fibroblast reorganization of substratum-bound fibronectin on surfaces varying in wettability, Journal of Biomedical Materials Research. 30 (1996) 385-391.

DOI: 10.1002/(sici)1097-4636(199603)30:3<385::aid-jbm13>3.0.co;2-j

[9] R. Derda, S. K. Y. Tang, A. Laromaine, B. Mosadegh, E. Hong, M. Mwangi, et al., Multizone Paper Platform for 3D Cell Cultures, PLoS ONE. 6 (2011) e18940.

DOI: 10.1371/journal.pone.0018940

[10] C. -I. Su, J. -X. Fang, X. -H. Chen, and W. -Y. Wu, Moisture absorption and release of profiled polyester and cotton composite knitted fabrics, Textile research journal. 77 (2007) 764-769.

DOI: 10.1177/0040517507080696

[11] K. -H. Kim, J. -S. Cho, D. -J. Choi, and S. -K. Koh, Hydrophilic group formation and cell culturing on polystyrene Petri-dish modified by ion-assisted reaction, Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 175–177 (2001).

DOI: 10.1016/s0168-583x(00)00650-9

[12] D. P. Dowling, I. S. Miller, M. Ardhaoui, and W. M. Gallagher, Effect of surface wettability and topography on the adhesion of osteosarcoma cells on plasma-modified polystyrene, J Biomater Appl. 26 (2011) 327-47.

DOI: 10.1177/0885328210372148

[13] J. Y. Lim, J. C. Hansen, C. A. Siedlecki, R. W. Hengstebeck, J. Cheng, N. Winograd, et al., Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: effect of nanotopography, surface chemistry, and wettability, Biomacromolecules. 6 (2005).

DOI: 10.1021/bm0503423