Characteristics of Biohydrogen Production by Mixed Culture Using Bagasse as the Substrate
Batch culture of dark fermentation was carried out to study the feasibility of biohydrogen production using bagasse as the substrate. In dark fermentation, hydrogen was produced by mixed culture using bagasse as the substrate. To establish favorable conditions for maximum hydrogen production, process parameters such as temperature and initial pH of the medium were investigated. Also, the component of biogas and liquid products of effluent by fermentation were analyzed by gas chromatography. The VFAs were mostly ethanol, acetic acid, propionic acid and butyric acid, and no valeric acid was observed. It is demonstrated that the hydrogen yield reached the maximum of 30.5mlH2/g bagasse while the temperature was 35°C in batch experiments under a series of temperature (25, 30, 35, 40°C) conditions. The initial pH ranged from 6.8 to 8.5, and the yield of hydrogen reached the maximum of 32mlH2/g bagasse with the initial pH of 8.5.
Zhenyu Du and X.B Sun
A. Y. Jiao et al., "Characteristics of Biohydrogen Production by Mixed Culture Using Bagasse as the Substrate", Advanced Materials Research, Vols. 113-116, pp. 1749-1754, 2010