In the present study we reported the feasibility of extracellular biopolymer(PF01) produced by Pseudomonas fluorescence as an alternative biosorbent to remove Cu(II) ions from environmental wastewater. The ability of PF01 to remove the Cu(II) ions was investigated by using batch biosorption procedure. The effects such as pH, dosage of biosorbent, Cu(II) initial concentration and sorbate–sorbent contact time on the adsorption were studied. The pseudo-second order kinetics and Langmuir isotherm models were noted be fit to the experimental data. The maximum Cu(II) adsorption capacity was 78.99 mg/g at pH 5.0 , at 28°C. The carboxyl , hydroxyl and amino groups of the PF01 were involved in chemical interaction with the Cu(II) ions depicted by Fourier transform infrared spectroscopic (FTIR). The study points to the potential of new use of extracellular biopolymer PF01 as an effective biosorbent for the removal of Cu(II) from environmental wastewater.