Optimization Process Protein Synthesis Culture in Five Alfalfa

Article Preview

Abstract:

The paper purposes is explore the forefront information of a large-scale protein production from alfalfa cells. With five varieties alfalfa, seleted the highest cell medium from callus . and detected the biomass and protein content in cell grows from different nitrogen source and sucrose concentration. Rusult is the highest protein in 12th -18th days of grows. There are different protein content in different nitrogen source (from Fig.2) and in different sucrose concentration range medium(from Fig.3).Conclusion is obtained the protein from alfalfal cells that is possibally.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Pages:

1918-1924

Citation:

Online since:

June 2010

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2010 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Lixuefeng,Wangning,Wushaohuan Apple Values Of Alfalfa and Prospect,Journal of Ningxia Agriculture College, No. 24, 2003 p.76~82.

Google Scholar

[2] Sujia-yi; Zhao Hongmei, Foreign artificial grassland,Grass-feeding livestock,No2, 2003, p.34~ 35.

Google Scholar

[3] Xu Zhanghui; Zhou Hongrong; Zhang Daming Induction and Regeneration System for Alfalfa Callus,An Hui Agriculture, No. 10, 2008,p.17~21.

Google Scholar

[4] OuYangYexin; LiZhongkui; LiuChenyun, Physiological and Biochemical Characterization of in Vitro Screened Rice Somaclonalo Variants Resistant to Xanthomonas Royse,ACTA PHYTOPATHOLOGICA SINIC, No. 01, 2000, p.113~118.

Google Scholar

[5] Wenhao Dai; and Cielo Castillo, Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species,Propagation and Tissue Culture, HortScience, Dec 2007; 42: 1670 - 1673.

DOI: 10.21273/hortsci.42.7.1670

Google Scholar

[6] Phil Bregitzer,Plant Regeneration and Callus Type in Barley: Effects of Genotype and Culture Medium,Crop Sci., Sep 1992; 32: 1108 - 1112.

DOI: 10.2135/cropsci1992.0011183x003200050007x

Google Scholar

[7] Lurline Marsh,116 Somatic Embryogenesis and Organogenesis in Cowpeas,HortScience, Jun 1999; 34: 461.

DOI: 10.21273/hortsci.34.3.461d

Google Scholar

[8] A Ben Amar, P Cobanov, K Boonrod, G Krczal, S Bouzid, A Ghorbel, and GM Reustle, Efficient procedure for grapevine embryogenic suspension establishment and plant regeneration: role of conditioned medium for cell proliferation. Plant Cell Rep, September 1, 2007; 26(9): 1439-47.

DOI: 10.1007/s00299-007-0341-8

Google Scholar

[9] Makoto Ikeda1, Kunihiko Ojima and Koji Ohira, Thiamine requirement of two different cultured cell lines of soybean,Plant Cell Physiol., Jun 1979; 20: 741 - 746.

DOI: 10.1093/oxfordjournals.pcp.a075864

Google Scholar

[10] Wang Liyan; Pei Zhongyou; Sun Shoujun, Influence of Different Media and Factors on Callus Induction Ratios in Sudan Grass 185,Journal of Tianjin Agricultural University, No. 04, 2006, p.24~ 25.

Google Scholar

[11] Cao Youlong; Xu Xin; Zhao Jun, Cell Suspension Culture Of Ephedra Intermeda Schrenket, Chinese Journal of Applied and Environmental Biology , No. 01, 2000, p.37~38.

Google Scholar

[12] Li Chun; Li Juan; Sheng Huihui, Metabolic Dynamic of Suspension Culture of Cistanche deserticola,Natural Product Research and Development , No. 19, 2007, p.193~194.

Google Scholar

[13] Luo Kai; Cha Xueqiang; Luo Jianping, Structure Kinetic Model of Maackia amurensis Cells in Suspension Culture , Chinese Journal of Biotechnology , No. 04, 2007, p.657~658.

Google Scholar

[14] Fang Wenjuan; Han Liebao; Zeng Huiming, Research Advances in Factors Affecting Establishment of Plant Cell Suspension Culture,Biotechnology Bulletin , No. 05, 2005, p.12~14.

Google Scholar

[15] Hou Wenxue; Zhen Suiping; Guo Yong, The Culturing Process and Kinetic Model Construction of Hibiscus sabdariffa Suspension Culture , Journal of Wuhan Botanical Research , No. 4, 2001, p.317~322.

Google Scholar

[16] Zhang Yi; Li Hongjian; Guo yong, Studies on Cell Culture and Superoxide Dismutase Production of Allium Sativum L. ,Journal of South China University of Technology(Natural Science Edition) , No. 3, 1993, p.92~93.

Google Scholar

[17] Li Yong; Xin Hui; Zhang Jinfang, Research on the Growth Regularity and Physiological Characteristics of Mulberry Suspension Cells,Science of Sericulture, No. 01, 2007, p.91~92.

Google Scholar

[18] Quchunxiang et. al, Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue, Journal of Suzhou university (Natural Science Edition)2(22), (2006).

Google Scholar