Identification of One Polysaccharide Gene and Analysis of Differentially Expressed Proteins from Bioflocculant Producing Strain of F2

Abstract:

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F2 is one of two compound bioflocculant producing strains with good genetic stability and high flocculating activity. The core sequence of exoL was amplified from the F2 genome by the method of polymerase chain reaction, identifying that the effective polysaccharide component of bioflocculant produced by F2 was succinoglycan. The changes of protein complexes of F2 grown in minimal media versus bioflocculant media were researched by SDS-PAGE, indicating that in bioflocculant media compared with minimal media, the relative content of two protein complexes were obviously increased and the relative content of one protein complexes was obviously decreased in gels. It was three differentially expressed protein complexes that can probably induce the production of large amount of the effective polysaccharide component of bioflocculant produced by F2. The research can provide a step to reveal the molecular flocculating mechanisms of compound bioflocculant producing strains.

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Edited by:

Zhenyu Du and X.B Sun

Pages:

305-310

DOI:

10.4028/www.scientific.net/AMR.113-116.305

Citation:

B. Yu et al., "Identification of One Polysaccharide Gene and Analysis of Differentially Expressed Proteins from Bioflocculant Producing Strain of F2", Advanced Materials Research, Vols. 113-116, pp. 305-310, 2010

Online since:

June 2010

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Price:

$35.00

[1] Fang Ma, Junliang Liu and Shugeng Li: Chinese Water and Wastewater. Vol. 19 (2003), pp.1-4.

[2] Qin Wang: The flocculation mechanism and production technology of the compound bioflocculant (Ph. D dissertation of Harbin Institute of Technology, China, 2005), pp.19-26.

[3] Lippolis J D, Bayles D O and Reinhardt T A: Journal of Proteome Research. Vol. 8 (2009), pp.149-158.

[4] Yanbin Zhu, Min Feng, Jixian Yang, Fang Ma, Bo Wu, Shugeng Li and Junli Huang: Journal of Harbin Institute of Technology, Vol. 36 (2004), pp.759-762.

[5] Glucksmann M A, Reuber T L and Walker G C: Bacteriol. Vol. 175 (1993), pp.7033-7044.

[6] T. Maniatis, E.F. Fritsch and J. Sambrook: Molecular cloning: a laboratory manual (Harbor Laboratory Press, America, 1982), pp.120-125.

[7] Bradford M M: Analytical Biochemistry. Vol. 72 (1976), pp.248-254.

[8] Wang W, Ma F, Yue XL and Wang AJ: The 2nd International Conference on Bioinformatics and Biomedical Engineering (ICBBE2008), Shanghai, China, May 16-18(2008), pp.1127-1130.

[9] Meng B, Qian Z, Wei F, Wang WW, Zhou CQ, Wang ZW, Wang QH, Tong W, Wang Q, Ma YH, Xu NZ and Liu SQ: Proteomics, Vol. 9 (2009), pp.3189-3200.

DOI: 10.1002/pmic.200800650

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