One Pot Green Synthesis of Gold Nanoparticles Using Piper Betle Leaf Extract and their Antibacterial Activities

Abstract:

Gold nanoparticles have been successfully synthesized using aqueous leaf extract of Piper betle In this extracellular synthesis, after exposing of metal ions to betel leaf extract, reduction leads into their metallic state and these are stabilized by the biomolecules present in leaf extract, where extract are being used as both reducing as well as stabilizing agents at ambient condition. Gold (AuNPs) nanoparticles are characterized by UV-Vis, FESEM, HRTEM and XRD measurements. Synthesized gold nanoparticles are mostly spherical in shape with diameter ~ 30-50 nm. Antibacterial activities of the synthesized nanoparticles are investigated against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive (Staphylococcus aureus and Bacillus thuringiensis) bacteria using the disc diffusion method. AuNPs show inhibition activity against P. aeruginosa and E. coli respectively nearly equivalent to the commercially available antibacterial drug e.g. Norfloxacin (Nx). The minimum inhibition concentration (MIC) results indicate that 36 μg/mL gold nanoparticles inhibit the growth of E. coli cells.

• Periodical: Advanced Materials Research (Volume 1163)
• Pages: 106-116
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1163.106
• Online since: April 2021
• Authors: Harekrishna Bar*
• Keywords: Antibacterial Activity, Gold Nanoparticles, Green Synthesis, MIC, Piper Betle

References

[1] P. Mohanpuria, N.K. Rana, S.K. Yadav, Biosynthesis of nanoparticles: technological concepts and future applications, J. of Nano Res. 10 (2008) 507–517.

DOI: 10.1007/s11051-007-9275-x

[2] A. Jagannathan, R. Rajaramakrishna, K.M. Rajashekara, J. Gangareddy, V. Pattar, S.V. Rao, B. Eraiah, V.J. Angadi, J. Kaewkhao, S. Kothan, Investigations on nonlinear optical properties of gold nanoparticles doped fluoroborate glasses for optical limiting applications, Journal of Non-Crystalline Solids, 53815 (2020) 120010.

DOI: 10.1016/j.jnoncrysol.2020.120010

[3] Y. Zou, Z. Guo, L. Ye, Y. Cui, K. Yu, Ultra-long cyclic Ni nanoparticles/carbon network hybrid lithium-ion battery anode toward smart electronics, J. of Allo. and Comp. 80330 (2019) 527-537.

DOI: 10.1016/j.jallcom.2019.06.271

[4] X. Xing, S. Tang, H. Hong, H. Jin, Concentrated solar photocatalysis for hydrogen generation from water by titania-containing gold nanoparticles, Int. J. of Hydro. Ener. 45(1620) (2020) 9612-9623.

DOI: 10.1016/j.ijhydene.2020.01.197

[5] Z. Ji, M.N. Ismail, Jr D.M. Callahan, E. Pandowo, Z. Cai, T.L. Goodrich, K.S. Ziemer, J. Warzywoda, Jr A. Sacco, The role of silver nanoparticles on silver modified titanosilicate ETS-10 in visible light photocatalysis, App. Cat. B: Env. 102 (2011) 323-333.

DOI: 10.1016/j.apcatb.2010.12.021

[6] F. Bayat, H. Tajall, Nanosphere lithography: the effect of chemical etching and annealing sequence on the shape and spectrum of nano-metal arrays, Heliyon, 6(2) (2020) e03382.

DOI: 10.1016/j.heliyon.2020.e03382

[7] L. Zou, R. Shen, L. Ling, G. Li, Sensitive DNA detection by polymerase chain reaction with gold nanoparticles, Analy. Chimi. Acta, 103814 (2018) 105-111.

DOI: 10.1016/j.aca.2018.07.006

[8] M. Šetka, F.A. Bahos, D. Matatagui, M. Potoček, S. Vallejos, Love wave sensors based on gold nanoparticle-modified polypyrrole and their properties to ammonia and ethylene, Sens. and Actu. B: Chem. 3041 (2020) 127337.

DOI: 10.1016/j.snb.2019.127337

[9] P. Mukherjee, R. Bhattacharya, N. Bone, Y.K. Lee, C.R. Patra, S. Wang, L. Lu, C. Secreto, P.C. Banerjee, M.J. Yaszemski, N.E. Kay, D. Mukhopadhyay, Potential therapeutic application of gold nanoparticles in B-chronic lymphocytic leukemia (BCLL): enhancing apoptosis, J. of Nanobiotech. 5 (2007) 4.

DOI: 10.1186/1477-3155-5-4

[10] A. Konefał, W. Lniak, J. Rostocka, A. Orlef, K. Rusiecka, Influence of a shape of gold nanoparticles on the dose enhancement in the wide range of gold mass concentration for high-energy X-ray beams from a medical linac, Reports of Practic. Onco. & Radiothe. 25(2020) 579-585.

DOI: 10.1016/j.rpor.2020.05.003

[11] D. Mandal, M.E. Bolander, D. Mukhopadhyay, G. Sankar, P. Mukherjee, The use of microorganisms for the formation of metal nanoparticles and their application, App. Microbio. and Biotech. 69 (2006) 485–492.

DOI: 10.1007/s00253-005-0179-3

[12] S. Balasubramanian, S.M.J. Kala, T.L. Pushparaj, Biogenic synthesis of gold nanoparticles using Jasminum auriculatum leaf extract and their catalytic, antimicrobial and anticancer activities, J. of Drug Deli. Sci. and Tech. 57 (2020) 101620.

DOI: 10.1016/j.jddst.2020.101620

[13] I. Kumar, M. Mondal, V. Meyappan, N. Sakthivel, Green one-pot synthesis of gold nanoparticles using Sansevieria roxburghiana leaf extract for the catalytic degradation of toxic organic pollutants, Mat. Res. Bull. 117 (2019) 18-27.

DOI: 10.1016/j.materresbull.2019.04.029

[14] D. Philip, Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis, Physica E. 42 (2010) 1417-1424.

DOI: 10.1016/j.physe.2009.11.081

[15] S.P. Dubey, M. Lahtinen, M. Sillanpaa, Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa, Coll. and Surf. A: Physicochem. Engin. Asp. 364 (2010) 34-41.

DOI: 10.1016/j.colsurfa.2010.04.023

[16] H. Bar, D.K. Bhui, G.P. Sahoo, P. Sarkar, S. Pyne, D. Chattopadhyay, A. Misra, Synthesis of gold nanoparticles of variable morphologies using aqueous leaf extracts of Cocculus hirsutus, J. of Exp. Nanosci. 7 (2012) 109-119.

DOI: 10.1080/17458080.2010.509875

[17] N.A. Begum, S. Mondal, S. Basu, R.A. Laskar, D. Mondal, Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts, Coll. and Surf. B: Biointerf. 71 (2009) 113-118.

DOI: 10.1016/j.colsurfb.2009.01.012

[18] M.P. Desai, G.M. Sangaokar, K.D. Pawar, Kokum fruit mediated biogenic gold nanoparticles with photoluminescent, photocatalytic and antioxidant activities, Proce. Biochem. 70 (2018) 188-197.

DOI: 10.1016/j.procbio.2018.03.027

[19] R.A. Zayadi, F.A. Bakar, M.K. Ahmad, Elucidation of synergistic effect of eucalyptus globulus honey and Zingiber officinale in the synthesis of colloidal biogenic gold nanoparticles with antioxidant and catalytic properties, Sustain. Chem. and Pharm. 13 (2019) 100156.

DOI: 10.1016/j.scp.2019.100156

[20] S. Vimalraj, T. Ashokkumar, S. Saravanan, Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties, Biomed. & Pharmacothera. 105 (2018) 440-448.

DOI: 10.1016/j.biopha.2018.05.151

[21] Y.C. Ko, Y.L. Huang, C.H. Lee, M.J. Chen, L.M. Lin, C.C. Tsai, Betel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan, J. of Oral Patholo. & Med. 24 (1995) 450–453.

DOI: 10.1111/j.1600-0714.1995.tb01132.x

[22] T. Nalina, Z.H.A. Rahim, The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans, Am. J. of Biochem. and Biotech. 3(2007) 10-15.

DOI: 10.3844/ajbbsp.2007.10.15

[23] S.B. Misra, S.N. Dixit, Antifungal activity of leaf extracts of some higher plants, Acta Botani. Ind. 7 (1979) 147-150.

[24] G. Santhanam, S. Nagarajan, Wound healing activity of Curcuma aromatica and Piper betle L, Fitoterapia, 61(1990) 458-459.

[25] P.H. Evans, W.S. Bowers, E.J. Vunk, Identification of fungicidal and nematocidal components in the leaves of Piper betle (Piperaceae), J. of Agri. and Food Chem. 32 (1984) 1254-1256.

DOI: 10.1021/jf00126a011

[26] S.J. Chen, B.N. Wu, J.L. Yeh, Y.C. Lo, I.S. Chn, I.J. Chen, C-fiber-evoked automatic cardiovascular effects after injection of Piper betle inflorescence extract, J. of Ethnophamaco. 45 (1995) 183-188.

DOI: 10.1016/0378-8741(94)01213-j

[27] W.D. Ratnasooriya, K.G.I. Jayawardena, G.A.S. Premakumara, Antimotility effects of Piper betle leaf extract on washed human spermatozoa, J. of the Nat. Sci. Found. of Sri Lanka, 18 (1990) 53-60.

DOI: 10.4038/jnsfsr.v18i1.8208

[28] S.A. Saeed, S. Farnaz, R.U. Simjee, A. Malik, Triterpenes and bsitosterol from Piper betle: isolation, antiplatelet and anti-inflammatory effects, Biochem. Soc. Trans. 21 (1993) 462S.

DOI: 10.1042/bst021462s

[29] I.C. Chopra, K.S. Jamwal, B.N. Khajuria, Pharmacological action of some common essential oil bearing plants used in indigenous medicine including P. betle, Indian J. Med. Res, 42 (1954) 385-389.

[30] M. Nagabhushan, A.J. Amonkar, U.J. Nair, A.V. D'Souza, S.V. Bhide, Hydroxy-Chavicol: a New Anti-Nitrosating Phenolic Compound from Betel Leaf, Mutagenesis 4 (1989) 200-204.

DOI: 10.1093/mutage/4.3.200

[31] N. Ramji, R. Iyer, S. Chandrasekaran, Phenolic antibacterials from Piper betle in the prevention of halitosis, J. of Ethnopharmaco. 83 (2002) 149-152.

DOI: 10.1016/s0378-8741(02)00194-0

[32] M. Sugumaran, M.S. Gandhi, K. Sankarnarayanan, M. Yokesh, M. Poornima, S.R. S.R. Rajasekhar, Chemical composition and antimicrobial activity of vellaikodi variety of Piper betle Linn Leaf oil against dental pathogens, Inter. J. of Pharm. Tech. Res. 3 (2011) 2135-2139.

[33] Q.L. Feng, J. Wu, G.O. Chen, F.Z. Cui, T.N. Kim, J.O. Kim, A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, J. of Biomed. Mat. Res. 52 (4) (2000) 662-668.

DOI: 10.1002/1097-4636(20001215)52:4<662::aid-jbm10>3.0.co;2-3