Covalent Immobilization of REDV Peptide on Heparin Coating Promotes Selective Adhesion of Endothelial Cells

Pei Xuan Zheng; Zhe Yu; Lian Duan; Jia Hao Zhang; Rao Fu; Rui Jiang

doi:10.4028/p-0tpt06

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1173Covalent Immobilization of REDV Peptide on Heparin...

Covalent Immobilization of REDV Peptide on Heparin Coating Promotes Selective Adhesion of Endothelial Cells

Abstract:

Forming an endothelial cell (EC) monolayer on the surface of blood-contacting devices is an ideal way to improve their blood compatibility, this process is known as endothelialization. Heparin is a proteoglycan with a strong negative charge, which is a commonly used antithrombotic agent. Heparin coatings also has been successfully used to improve the hemocompatibility of various biomaterials. In this study, a short peptide Arg-Glu-Asp-Val (REDV) with specific affinity for endothelial cells was covalently conjugated with maleimide-functionalized heparin. The conjugate retains the negative charge of heparin and can be directly immobilized on the surface of positively charged poly-L-lysine. This specific reaction to the carboxyl group of heparin could potentially reduce the shedding and steric hindrance effects of REDV peptide and enhancing the affinity and selectivity of heparin for EC.

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Periodical:

Advanced Materials Research (Volume 1173)

Pages:

85-89

DOI:

https://doi.org/10.4028/p-0tpt06

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Online since:

August 2022

Authors:

Pei Xuan Zheng, Zhe Yu, Lian Duan, Jia Hao Zhang, Rao Fu, Rui Jiang*

Keywords:

Endothelial Cells, Endothelialization, Heparin, REDV Peptide

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* - Corresponding Author

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