Response Surface Methodology: An Optimization of Process Variables for the Nanoencapsulation of Anthocyanins from Black Rice Bran

Abstract:

Nanoencapsulation technology has been used in food and pharmaceutical applications to increase bioactive chemical functioning and stability against external influences. To develop a cost-effective encapsulating procedure, additional optimization is required. This study employed response surface methodology (RSM) to optimize the encapsulation of anthocyanin-rich extract from black rice bran. The extract was encapsulated through pre-gelation and polyelectrolyte complex formation processes. Box-Behnken design was employed to determine the optimum conditions for the encapsulation process with the following process variables: chitosan concentration, pH, and CaCl₂ concentration. Chemical characteristics, surface morphology, and particle size were used to describe the resultant capsules, which were then subjected to phytochemical analysis. The optimal encapsulation conditions for anthocyanin were 6.30 mg/mL chitosan, pH 5.5, and 36 mM CaCl_2, with a 51.20 % encapsulation efficiency. The developed anthocyanin-loaded nanocapsule has a high TPC (3.87 mg GAE/g) and potent antioxidant activity (5.69 mg TE/g). SEM images revealed a smooth surface area and spherical particles that clumped together, with an average particle size of 94.70 nm. FTIR analysis corroborates the well-incorporation of anthocyanin into the nanocapsules. The encapsulation process of anthocyanin-rich extract from black rice bran was successfully optimized via RSM.

• Periodical: Advanced Materials Research (Volume 1179)
• Pages: 95-107
• DOI: https://doi.org/10.4028/p-rV054a
• Online since: January 2024
• Authors: Rodel M. Bulatao, Paul Jhon G. Eugenio*, John Paulo A. Samin, Joel R. Salazar, Juvy J. Monserate
• Keywords: Anthocyanin, Black Rice Bran, Box-Behnken Design, Nanoencapsulation, Response Surface Methodology

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