The properties of composites reinforced by three dimensional braided preform are determined by braiding structure significantly. The main objective of this paper is to develop a general methodology for the determination of the design and analysis of three dimensional two-step braided tubular preform. The arrangement pattern of axial yarns with various finenesses is derived for the uniform braiding structure of preform, which offers a possibility for achieving preferable interior structures of braided tubular preforms. Then, the general structure parameters, including the interrelation between surface braiding angles and interior braiding angles and the fiber volume fraction, are investigated in some detail. The results derived from this paper can provide a useful method for the design of 3D two-step tubular braided preform.