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Paper Titles
Preface
The Fluorescent Pigment of Mulberry Transferred in the Body of Silkworm, Bombyx Mori
p.3
Application of Pebrine Detection by PCR Infected Bombyx Mori Eggs
p.8
Cloning and Expression Analysis of Acetylcholinesterase Gene (Bm-ace1, Bm-ace2) from Domesticated Silkworm, Bombyx mori
p.13
Transcriptional Expression of Serpin-6 Gene in Bombyx Mori Larval after Infection of Bacteria at Different Developmental Stages
p.19
Separation and Identification of Proteins Related to Fruits Ripening in Mulberry (Morus alba)
p.25
Differential Expression Analysis of Fat Body Proteome during Pupation in Silkworm (Bombyx mori)
p.30
Determination of DNJ of Mulberry Latex and Evaluation of the Hypoglycemic Effect on Mice
p.36
Studies on the Joint Impact of Mulberry Cultivation and Sericulture Enterprise as a Scheme for Border Area Development Programme in Kashmir, India
p.41

Transcriptional Expression of Serpin-6 Gene in Bombyx Mori Larval after Infection of Bacteria at Different Developmental Stages

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Abstract:

Serpins can block different steps in the activation cascade of prophenoloxidase (proPO) system, and play an important role in immunity of insect. In this paper, Haemolymph was collected from the 4th molting, newly moulted 5th instar and day-3 fifth instar larval challenged by lipopolysaccharide (LPS) or Bacillus thuringiensis, respectively. The results revealed that the transcriptional level of Bmserpin-6 in different developmental stages showed a trend of rise first, then fall. Bmserpin-6 of the 4th molting larva expressed highest at 6h post-infection with LPS and 3h post-infection with Bacillus thuringiensis. Bmserpin-6 of newly moulted 5th instar larva expressed highest at 9h post-infection with LPS and 6h post-infection with Bacillus thuringiensis. Bmserpin-6 of day-3 fifth instar larva expressed highest at 9h post-infection with Bacillus thuringiensis. Bmserpin-6 was all highly induced and highly expressed in haemolymph of larval at different developmental stages. But The time to arrive the highest transcriptional level was different. This is inferred that the Serpin gene may play an important role in immunity of Bombyx mori.

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Periodical:

Advanced Materials Research (Volumes 175-176)

Pages:

19-24

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.175-176.19

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Online since:

January 2011

Authors:

Hong Xian Zha, Yan Fang Yu, Yan Yun Wang, Shan Shan Sun, Zheng Guo Wei, Bing Li, Yu Hua Chen, Ya Xiang Xu, Wei De Shen

Keywords:

Bombyx mori, Immunostimulatory, Semi-Quantitative RT-PCR, Serpin-6, Transcription

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© 2011 Trans Tech Publications Ltd. All Rights Reserved

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