Application of Pebrine Detection by PCR Infected Bombyx Mori Eggs

Zhong Hua Pan; Cheng Liang Gong; Xiao Jian Zheng; Rui Guo; Wei De Shen

doi:10.4028/www.scientific.net/AMR.175-176.8

Paper Titles

The Fluorescent Pigment of Mulberry Transferred in the Body of Silkworm, Bombyx Mori
p.3

Application of Pebrine Detection by PCR Infected Bombyx Mori Eggs
p.8

Cloning and Expression Analysis of Acetylcholinesterase Gene (Bm-ace1, Bm-ace2) from Domesticated Silkworm, Bombyx mori
p.13

Transcriptional Expression of Serpin-6 Gene in Bombyx Mori Larval after Infection of Bacteria at Different Developmental Stages
p.19

Separation and Identification of Proteins Related to Fruits Ripening in Mulberry (Morus alba)
p.25

Differential Expression Analysis of Fat Body Proteome during Pupation in Silkworm (Bombyx mori)
p.30

Determination of DNJ of Mulberry Latex and Evaluation of the Hypoglycemic Effect on Mice
p.36

Studies on the Joint Impact of Mulberry Cultivation and Sericulture Enterprise as a Scheme for Border Area Development Programme in Kashmir, India
p.41

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 175-176Application of Pebrine Detection by PCR Infected...

Application of Pebrine Detection by PCR Infected Bombyx Mori Eggs

Abstract:

With the development of the PCR technology, especially the improvement of its reagent and a method of pebrine detection by PCR in infected Bombyx mori eggs was established.With the 16sRNA gene of Nosema bombycis as target sequence, the results of extraction of genomic DNA from purified microspores showed that 1.3×10-7µg DNA can be extracted from each spore. The sensitivity detection showed the detection limit of Nosema bombycis DNA was 3.25×10-2pg, i.e. 4 spores. (PCR system volume is 25µl). The method of total DNA extraction from pebrine infected silkworm eggs just before hatching was created. The result showed that extracting total DNA from silkworm eggs after the eggs had been treated with 30% KOH met the PCR detection requirements. The result of application study showed the spores in the pebrine infected egg just before hatching can sensitively be found with PCR. The result of a group of eggs just before hatching detection showed that the maximum PCR detection level was of a pebrine infected eggs just before hatching in 300 healthy eggs when the total DNA extraction had been purified with Agarose electrophoresis. The probability of identifying groups of one pebrine spore in infected eggs just before hatching mixed with 100 healthy ones was about 80%.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 175-176)

Pages:

8-12

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.175-176.8

Citation:

Cite this paper

Online since:

January 2011

Authors:

Zhong Hua Pan, Cheng Liang Gong, Xiao Jian Zheng, Rui Guo, Wei De Shen

Keywords:

Application Study, PCR Detection, Pebrine Infected Bombyx Mori Eggs

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] X.Chen, K. W. Huang and Z.Y. Shen: Acta Sericologica Sinica Vol.4 (1996), p.229

Google Scholar

[2] P. Z. Cai, X. Y. Xu and Z. R. Huang: Acta. Sericologica Sinica Vol. 4 (1997), p.207

Google Scholar

[3] B. L. Qiu, X. Y. XU and Z.M. MU: Journal of Shandong Agricultural University Vol.1 (2002), p.14

Google Scholar

[4] J. P. Liu, Y. Cao and J. E. Smith: Acta Sericologica Sinica Vol. 4 (2004), p.367

Google Scholar

[5] Y. Hatakeyama, S. Hayasaka S: JARQ Vol 36 (2)(2002), p.97

Google Scholar

[6] R.H. Liu, Q. B. Liu and Y.J. Wan: Hybrids Bulletin of Sericulture Vol. 40 (3) (2003), p.86

Google Scholar

[7] J.P. Liu, Y. Cao, J.E. Smith: Scientia Agricultura Sinica Vol. 44 (4)(2004), p.53

Google Scholar

[8] Z.H. Pan, X. J. Zheng, and R.Y. Xue: Acta Sericologica Sinica Vol. 48 (2)(2005), p.90

Google Scholar