Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

Li Li Feng; Jian Fei Zhang; Hui Luo; Zheng Li; Hong Jie Zhang

doi:10.4028/www.scientific.net/AMR.175-176.192

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 175-176Study on Optimization of Fermentation Condition...

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

Abstract:

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

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Periodical:

Advanced Materials Research (Volumes 175-176)

Pages:

192-196

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.175-176.192

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Online since:

January 2011

Authors:

Li Li Feng, Jian Fei Zhang, Hui Luo, Zheng Li, Hong Jie Zhang

Keywords:

Acetonitrile, Escherichia Coli BL21 (DE3)/pET-Nit, Nitrilase, Optimum

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