Synthesis and Characterization of Novel Silk-Like Proteins Using Genetic Engineering Methods

Ming Ying Yang; Liang Jun Zhu; Si Jia Min; Tetsuo Asakura

doi:10.4028/www.scientific.net/AMR.175-176.258

Paper Titles

Inflammatory Response of Native Silk Fibroin Powder/Polyurethane Composite Membrane Containing Aspirin In Vivo
p.236

Study on Structures of Electrospinning Cellulose Acetate Nanofibers
p.242

Research on Water Resistance of Polyvinyl Alcohol Nanofiber Mats
p.247

Studies on Recombinant Human Bone Morphogenetic Protein 2 Loaded Nano-Hydroxyapatite/Silk Fibroin Scaffolds
p.253

Synthesis and Characterization of Novel Silk-Like Proteins Using Genetic Engineering Methods
p.258

Study on Improving Biodegradation Process of Regenerated Silk Fibroin Fiber
p.266

Wet-Spinning of Reinforced Artificial Silk Hybrid Fibres by Cellulose Whiskers
p.272

A Hydrophobic Bombyx Silk Preparation Using Sol-Gel Techniques
p.276

Effects of Heat Shrinkage on Structure and Property of Silk/Synthetic Fiber Textured Composite Filaments
p.280

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 175-176Synthesis and Characterization of Novel Silk-Like...

Synthesis and Characterization of Novel Silk-Like Proteins Using Genetic Engineering Methods

Abstract:

Using genetic engineering methods, we attempted to produce novel silk-like proteins with new function by combining several functional sequences selected from fibroin of Bombyx mori (B.mori), Samia Cynthia ricini (S.c.ricini) and spider silks or by inducing cell adhesive sequence or calcium binding sequence into silk proteins. The secondary structure of these silk-like proteins was characterized with solid state NMR. Cell adhesion assay indicated that silk-like proteins have higher cell activity. Mineralization of fibroin protein was improved with induction of calcium binding sequence. Nanofiber formation of silk-like proteins was achieved using electrospinning. Fiber was formed from silk-like proteins. These silk-like proteins might be candidates to meet requirement in the field of biomaterials.

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Periodical:

Advanced Materials Research (Volumes 175-176)

Pages:

258-265

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.175-176.258

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Online since:

January 2011

Authors:

Ming Ying Yang, Liang Jun Zhu, Si Jia Min, Tetsuo Asakura

Keywords:

Cell Adhesion Activity, Electro-Spinning, Fiber Formation, Genetic Engineering Methods, Mineralization, Silk-Like Proteins, Structure Characteristics

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