In order to develop silk underwear fabric with good anti-bacterial property, silk was grafted using 2-diethylaminoethyl methacrylate (DEAEMA) by atom transfer radical polymerization (ATRP) method. The amino groups and hydroxyl groups on the side chains of the silk fibroin was reacted with 2-bromoisobutyryl bromide (BriB-Br) to obtain efficient macroinitiator for ATRP. And the macroinitiator was grafted with DEAEMA in water/ethanol media using CuBr/N,N,N',N",N" -pentamethyldiethylenetriamine (PMDETA) as catalyst system. The effects of monomer concentration, the proportion of CuBr and PMDETA, grafting temperature and time on the silk grafting were discussed, and the optimal grafting technology was obtained. FT-IR characterization of the grafted silk showed a peak corresponding to DEAEMA, which indicated that DEAEMA was grafted onto the surface of silk. The quaternized grafted silk had good anti-bacterial property to S. aureus and E. coli. . And the bacteria repellency of the grafted silk had good washing durability.