A Method Suitable for Total Protein Extraction from Wood Forming Tissue of Pinus koraiensis

Abstract:

Total protein extraction method should be adjusted for get higher quality product for special material. We optimized the protocols and extracted total proteins from P. koraiensis adopt TCA-acetone, TCA-acetone contains β-mercaptoethanol, methanol and protein extraction kit respectively. Then we compared extracts from these methods using SDS-PAGE. The results show that there are no obvious differences between TCA, TCA-2ME and Methanol, which all have better bands. In contrast, extracts from protein extraction kit had ambiguous bands. We also found that it should take about eight hours at least for precipitate of mixtures of samples and extraction solutions. We think optimized TCA-acetone is an idea method for protein extraction from P. koraiensis.

• Periodical: Advanced Materials Research (Volumes 179-180)
• Pages: 1199-1202
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.179-180.1199
• Online since: January 2011
• Authors: Jiang Tao Shi, Jian Li
• Keywords: Pinus koraiensis, Protein Extraction, TCA-Acetone, Wood Forming Tissue

References

[1] Plomion C., et al. Plant Physiology. December 2001, Vol. 127, pp.1513-1523.

[2] Plomion C., et al. Plant Physiol. July 2000, Vol. 123, pp.959-970.

[3] Gion J. M, et al. Proteomics. 2005, 5: 3731-3751.

[4] Hertzberg M, et al,. Proc Natl Acad Sci USA 2001, 98: 14732-14737.

[5] J.A.P. Paiva, et al,. New Phytologist. 2008, 178: 283-301.

[6] J.A.P. Paiva, et al,. New Phytologist. 2008, 179: 1080-1094.

[7] Richard J. Simpson. Proteins and Proteomics: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (2003).

DOI: 10.1086/380006

[8] Damerval, et al. Electrophoresis. 1986, 7, 52-54.

[9] Aki. T, et al. Plant Cell Physiol. May 2008, 49(5): 767-790.

[10] Mechin V, Damerval C, Zivy M. Total protein extraction with TCA-acetone. Methods Mol Biol 2007; 355: 1-8.

DOI: 10.1385/1-59745-227-0:1