Poly-β-hydroxybutyrate (PHB) is a kind of polyesters. Due to its biodegradability and other extraordinary properties, PHB could be ideal material for use in medicine, pharmacy, food packaging and agriculture. Of the possible carbon sources, methane could be proved to be one of the most suitable substrates to the production of PHB. The experiment is about a serious of studies that methanotrophic strain IMV M3011 、M3021、and GYJ3 use methane as carbon to accumulate PHB, and their cell growth and PHB accumulation are studied. The capacity of producing PHB about three kinds of Methanotrophic strains is compared. As a result, methanotrophic strain IMV M3011 has a highest capcity to growth at 144h, the biomass of the cell is 1.22g/L, the secondly is M3021, and the GYJ3 is lowest, and methanotrophic strain IMV M3011 also has a highest capcity to produce PHB, PHB content is 16.94%, the secondly is M3021, The GYJ3 is lowest.