Through the analysis of wood microstructure, the study revealed the reason of forming the wood zone line pattern, and analyzed the wood zone line pattern’s similarities and differences between the artificial cultivation and natural formation. And the wood zone line pattern was formed because that the fungal hyphae invaded into the wood inside. The research results showed that: (1) The causes for the formation of wood zone line pattern, took place when different fungi eroding the same tree, due to antagonism among the hyphae, and the zone line was formed on their against interface. (2) Artificial cultivation and natural formation wood zone line pattern were the same, their hyphae both entered into the wood cells, and formed the irregular, natural and beautiful pattern. The wood zone line pattern could be used as the woodwork surface decoration materials, they were the excellent materials of furniture and indoor decoration. The study laid a theoretical foundation for further research on artificial cultivation fungi coloring wood, really realized the wood biological coloring. And the wood zone line pattern was green, low-carbon, and environmentally friendly products.