We investigate the influence of grain size on mechanical properties in a Fe-9Ni-12Mn-2.5Si-1.0C TWIP steel by unidirectional tensile. Meanwhile the microstructures of the TWIP steel were observed and analyzed by optical microscope (OM) and transmission electron microscope (TEM). The experimental results show that the TWIP steel’s yield strength and tensile strength decrease with the increasing of grain size, whereas the plasticity increases with it. When the average grain size reaches to 27μm, the tensile strength is 1080MPa, the elongation percentage is 77%, and the strength-plasticity product achieves the 83160MPa•%. Steel’s strain hardening rate can be changed from three-stage to four-stage with the increasing of grain sizes, the areas of strain hardening by twin deformation mechanism are expanded. Through the microstructure observation we found that, coarse-grained TWIP steel conducts to twinning formation, the high density twins can increase the alloy’s ductility by splitting the grain.