PEM fuel cell is an ideal power for modern automobile. Characteristics distribution of PEM fuel cell is one of key parameters for its application. Reactant gas stoic ratio, current density and charge capacity are measured by the current distribution measurement system, and their distribution and the influence of load on them are analyzed in this paper. Experimental results show that the reactant stoic ratio and charge capacity decrease along the gas channel; the optimal humidification temperature decreases with the increase of external load. These results are very helpful for the optimization of operating parameters of PEM fuel cell and its application.