Effects of Antioxidant Enzymes of Ascorbate-Glutathione Cycle in Soybean (Glycine Max) Leaves Exposed to Ozone

Abstract:

Open-top chambers (OTCs) were used to investigate the mechanism of antioxidant enzymes to eliminate reactive oxygen species (ROS) of plants under troposphere O₃ stress. The results indicated that, compared to control, the O₃ concentration of 80±10 nL·L^-1 and 110±10 nL·L^-1 induced an increase on malondialdehyde (MDA) content and a decrease on superoxide anion (O₂) production rate and hydrogen peroxide (H₂O₂) content during the whole growth stage. Simultaneity, it showed a trend of increasing in earlier stage and decreasing in later stage of the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), while the dehydroascorbate reductase (DHAR) activity was increased in earlier period, decreased in middle periods and then increased in later period compared to control, respectively. The results show that elevated O₃ concentration accelerates ROS metabolism rates, reduces the efficiency of antioxidant enzymes that can not tolerate oxidative damage caused by elevated O₃ concentration, which represents injured affects to soybean.

• Periodical: Advanced Materials Research (Volumes 204-210)
• Pages: 672-677
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.204-210.672
• Online since: February 2011
• Authors: Tian Hong Zhao, Jun Li Wang, Yan Wang, Ying Cao
• Keywords: Antioxidant Enzyme, Ozone, Reactive Oxygen Species, Soybean

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