Molecular Characterization and B-Cell Epitopes Prediction of the Antigen pEtK2 of Eimeria Tenella
Avian coccidiosis，caused by parasites of the genus Eimeria, is a major intestinal disease of chickens. In order to develop a new generation of vaccine which can provide a safer, more effective and cost-effective approach for avian coccidiosis control, bioinformatic and molecular approaches are used to predict and analyze the molecular characterization and B-cell epitopes of Eimeria antigens. In the present study, the molecular characterization and B-cell epitopes of the antigen pEtK2 of Eimeria tenella（E.tenella）were predicted by using of bioinformatics techniques. The pEtK2 gene was predicted to encode a polypeptide of 487 amino acids with a putative molecular mass of 55.102 kDa , and a predicted isoelectric point of 5.707. The acid sequence analysis showed that the potential antigenic epitopes are situated in 1-19,51-70,135-154,237-255,279-299 and 420-439 amino acids. The results obtained from the present study may provide necessary data for further study of E.tenella pEtK2 and development of recombinant vaccines and epitope vaccines.
Helen Zhang, Gang Shen and David Jin
X. W. Chen et al., "Molecular Characterization and B-Cell Epitopes Prediction of the Antigen pEtK2 of Eimeria Tenella", Advanced Materials Research, Vols. 219-220, pp. 1569-1573, 2011