The adsorption of dimethylformamide (DMF) by macroporous chitosan membranes using silica gel as porogen is studied. The morphology, porosity and DMF adsorption capacity of the macroporous chitosan membrane were measured. SEM photographs show the pores in the membrane dispersed uniformly. DMF adsorption capacity of the macroporous chitosan membranes reached 145 mg/g. Adsorption isotherm of DMF on the macroporous chitosan membranes was determined and correlated with Langmuir and Freundlich equations. The adsorption equilibrium data fitted well with Freundlich equation. The adsorption kinetics was found to follow the pseudo second-order kinetic model.