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Breeding of Xylanase-Producing Strains by Ion Implantation and Studying of Fermentation Conditions

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Abstract:

With ion implantation, a high xylanase-producing strain Aspergillus niger BU99 was selected. Different late acting carbon sources (wheat bran, wheat straw, corncob and bagasse) had an effect on enzymatic production. Additionally, it was found that fast acting carbon sources (lactose, galactose, sucrose, glucose and maltose) put into the media affected on xylanase production too. Severe catabolic repression was observed in the media with lactose, but other sugars (galactose, sucrose, glucose and maltose) could increase the production of xylanase compared with the control. The production of xylanase was improved too when corncob flour was hydrolyzed by α-amylase, cellulase or/and glucoamylase. Additionally, it was found that the production of xylanase would be almost higher at 60h when the media contained glucose or were hydrolyzed by enzymes.

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Periodical:

Advanced Materials Research (Volumes 233-235)

Pages:

1442-1446

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.233-235.1442

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Cite this paper

Online since:

May 2011

Authors:

Shi Chang Li, Dong Dong Wang, Zhao Yang Zhu, Hong Xia Liu

Keywords:

Aspergillus Niger BU99, Fast Acting Carbon Sources, Ion Implantation, Late Acting Carbon Sources, Submerged Liquid Cultivation, Xylanase

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© 2011 Trans Tech Publications Ltd. All Rights Reserved

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