A Simple and Convenient Method for Simultaneous Determination of Oleic and Linoleic Acid in Coix Seed Oil
A simple and convenient method for simultaneous determination of two major effective components (oleic acid and linoleic acid) in Coix seed oil has been developed. The sample was analyzed in a RP-C18 column with acetonitrile: tetrahydrofuran: water (containing 0.4% HAC) (77:3:20, v/v/v) as a mobile phase, and the differential refractive index detector was used in HPLC. The column temperature was fixed at 30°C and the flow rate was 1.0 ml/min. The average recovery rate of oleic acid and linoleic acid are 102.83% and 99.33%, the RSD (n=6) are 1.24% and 2.27%, respectively. The standard curves of oleic acid and linoleic acid presented the favorable linear correlation over the range of 0.098-10.05 mg/ml and 0.101-10.38 mg/ml(R2 >0.999). The method reported in this paper is reliable and relatively simple with a good reproducibility for the determination of oleic acid and linoleic acid in Coix seed oil comparing with the existing methods.
Zhong Cao, Lixian Sun, Xueqiang Cao, Yinghe He
X. N. Xiao et al., "A Simple and Convenient Method for Simultaneous Determination of Oleic and Linoleic Acid in Coix Seed Oil", Advanced Materials Research, Vols. 233-235, pp. 2629-2632, 2011