A Simple and Convenient Method for Simultaneous Determination of Oleic and Linoleic Acid in Coix Seed Oil

Xiao Nian Xiao; Hai Bin Yi; Xing Yi; Hua Xiong

doi:10.4028/www.scientific.net/AMR.233-235.2629

Paper Titles

Research on Reaction Mechanism of SiC and AlF₃ in CO Atmosphere
p.2610

A Three-Dimensional Transient Model of Two-Phase Mass Transfer in Anode Side of Passive Direct Methanol Fuel Cells
p.2615

Fluorescence Spectroscopic Studies of Interaction between 4-Aminobenzenesulfonic Acid and Bovine Serum Albumin
p.2619

The Co-Doping Effect of Si and Mn on the Dilute Ferromagnetic Semiconductor Thin Films
p.2624

A Simple and Convenient Method for Simultaneous Determination of Oleic and Linoleic Acid in Coix Seed Oil
p.2629

Corrosion Behavior of 10CrNiCu Steel Influenced by Thiobacillus Ferrooxidans
p.2633

Needle-Like Mullite Ceramic Prepared by Sol-Gel Process
p.2640

Industrial Experiments of Using Limestone Instead of Lime for Slagging during LD-Steelmaking Process
p.2644

Discussion on the Decomposition Laws of Limestone during Converter Steelmaking Process by Static Decomposition Model under Constant Temperature
p.2648

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 233-235A Simple and Convenient Method for Simultaneous...

A Simple and Convenient Method for Simultaneous Determination of Oleic and Linoleic Acid in Coix Seed Oil

Abstract:

A simple and convenient method for simultaneous determination of two major effective components (oleic acid and linoleic acid) in Coix seed oil has been developed. The sample was analyzed in a RP-C18 column with acetonitrile: tetrahydrofuran: water (containing 0.4% HAC) (77:3:20, v/v/v) as a mobile phase, and the differential refractive index detector was used in HPLC. The column temperature was fixed at 30°C and the flow rate was 1.0 ml/min. The average recovery rate of oleic acid and linoleic acid are 102.83% and 99.33%, the RSD (n=6) are 1.24% and 2.27%, respectively. The standard curves of oleic acid and linoleic acid presented the favorable linear correlation over the range of 0.098-10.05 mg/ml and 0.101-10.38 mg/ml(R2 >0.999). The method reported in this paper is reliable and relatively simple with a good reproducibility for the determination of oleic acid and linoleic acid in Coix seed oil comparing with the existing methods.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 233-235)

Pages:

2629-2632

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.233-235.2629

Citation:

Cite this paper

Online since:

May 2011

Authors:

Xiao Nian Xiao, Hai Bin Yi, Xing Yi, Hua Xiong

Keywords:

Coix Seed Oil, High Performance Liquid Chromatography (HPLC), Linoleic Acid, Oleic Acid, Simultaneous Determination

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Feng Lu, Yanmei Jiang. Caixia Yang. Journal of Nutrition, 2008, 30(1): 102-105. (In Chinese)

Google Scholar

[2] Xiaorong Wen. Traditional Chinese Medicine Journal of Liao Ning University, 2008, 10(3):135-138. (In Chinese)

Google Scholar

[3] Changsheng Li, Yun Lu. Medicine Journal of Shan Xi, 2007, 36 (12):1682-1684. (In Chinese)

Google Scholar

[4] Dapeng Li. China's new drugs and clinical magazines, 2007, 26 (10):778-782. (In Chinese)

Google Scholar

[5] Chengyou Ke, Xiaofang Wu. Chinese Journal of Health Laboratory Technology, 2005, 15 (5):528-530. (In Chinese)

Google Scholar

[6] Xu Lei, Tong Zhang. Journal of Hebei Medical University, 2007, 28 (3):237-240. (In Chinese)

Google Scholar

[7] Hong Li, Changlin Yue, Qi Xiang, et al. Application of modern Chinese medicine Journal, 2006, 23 (4): 329-331. (In Chinese)

Google Scholar

[8] Ding Yi, Xing Tang. Drug Analysis magazine, 2004, 24 (3) :249-252. (In Chinese)

Google Scholar