Fluorescence Spectroscopic Studies of Interaction between 4-Aminobenzenesulfonic Acid and Bovine Serum Albumin

Abstract:

The interaction between 4-aminobenzenesulfonic acid and bovine serum albumin (BSA) had been studied by fluorescence spectroscopy. The binding constant (K=2.51×10⁴) and binding points (n=0.998) of 4-aminobenzenesulfonic acid with BSA were determined at 298 K based on fluorescence quenching results. The thermodynamic parameters such as enthalpy change (ΔH), entropy change (ΔS) and Gibbs free-energy change (ΔG) for the reactions were also calculated according to the thermodynamic equations. The negative ΔH and ΔS values in case of 4-aminobenzenesulfonic acid-BSA complexes showed that van der Waals interactions and hydrogen bonds might play a major role in the binding of 4-aminobenzenesulfonic acid to BSA. The distance, r, between donor (BSA) and acceptor (4-aminobenzenesulfonic acid) was obtained according to the Förster’s theory of non-radiation energy transfer. The results of synchronous fluorescence spectra revealed that 4-aminobenzenesulfonic acid induced conformational change of BSA.

• Periodical: Advanced Materials Research (Volumes 233-235)
• Pages: 2619-2623
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.233-235.2619
• Online since: May 2011
• Authors: Jian Li Liu, Xiang Yu Cao, Xiu Li Lu, Ya Peng Huo
• Keywords: 4-Aminobenzenesulfonic Acid, Bovine Serum Albumin (BSA), Fluorescence Spectroscopic Studies, Interaction

References

[1] Y.Z. Zhang,B. Zhou, X.P. Zhang, P. Huang, C.H. Li and Y. J. Liu: Hazard. Mater. Vol. 163 (2009), p.1345

[2] J. Flarakos, K.L. Morand and P.Vouros :Anal. Chem. Vol.77(2005), p.1345

[3] T.O. Hushcha, A.I. Luik, and Yu.N. Naboka :Talanta. 53(2000), p.29

[4] P. Singh, L.C. Mishra, A., Pandey and L. Lyengar: Bioresour. Technol. 97(2006), p.1655

[5] O. Zerbinati, M. Vincenti, S. Pittavino and M.C. Gennaro: Chemosphere. 35(1997), p.2295

DOI: 10.1016/s0045-6535(97)00308-1

[6] J.B. Xiao, J.W. Chen, H. Cao, F.L. Ren, C.S. Yang, Y. Chen and M.Xu: J. Photochem. Photobiol., A. 191(2007),p.222

[7] P.N. Naik, S.A. Spectrochim: Acta, Part A. 73(2009), p.841

[8] P.B. Kandagal, J. Seetharamappa, S. Ashoka, S.M.T. Shaikh, D.H. Manjunatha: Int. J. Biol. Macromol. 39(2006), p.234

[9] X.J. Guo, X.W. Han, J. Tong, C. Guo, W.F. Yang, J.F. Zhu and B.Fu: J. Mol. Struct. 966(2010), p.129

[10] P.D. Ross and S. Subramanian: Biochemistry. 20(1981), p.3096

[11] A. Mallick, B. Haldar, N. Chattopadhyay: J. Phys. Chem. B. 108(2005), p.14683

[12] Föster, T. Ann, Phys. 2(1948), p.55

[13] J. Yang, Z.H. Jing, J.J. Jie, P.Guo: J. Mol. Struct. 920(2009), p.227