Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip

Zhong Cao; Li Xian Sun; Ting Zhou; Yong Feng Luo; Ju Lan Zeng; Shu Long; Ji Shan Li

doi:10.4028/www.scientific.net/AMR.236-238.1923

Paper Titles

A Novel Way to Synthesize Mesoporous Silica Coated on the CNTs with Larger Holes Based on “MCM-41” CNTs@SiO₂
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Mineralization of a Bioactive Si-Ca-P System Wet Gel in Aqueous K₂HPO₄ Solution
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Study on Preparation, Biological Activity and Thermal Decomposition of N-Benzoyl-N＇-(4-Chlorobenzamido) Thiourea
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The Competitive Growth of BiOI and BiSI in the Solvothermal Process
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Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip
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Formation and Magnetic Characterization of Magnesium Oxide / Iron Nano Composite Particles
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Gold Magnetic Particle Based Immunoassay on a Disposable Microchips
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Reduction of Supercooling of Water by TiO₂ Nanoparticles as Nucleating Agent
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Effects of Annealing on Microstructure and Mechanical Properties of Bulk Nanocrystalline Fe3Al Based Alloy with 10 Wt. % Mn Prepared by Aluminothermic Reaction
p.1939

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 236-238Investigation on Electronic Signals for Detection...

Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip

Abstract:

An electronic detection method for DNA molecules based on an extended gate field effect transistor (EGFET) sensing chip has been presented in this paper, which consists of one gold plate electrode for molecule recognition and FET part for signal transduction. The DNA probe was prepared by first immobilization of a thiolated single-stranded oligonucleotide (T1) and then an alkanethiol such as 6-hydroxy-1-hexanethiol (6-HHT) on the gold plate. A fast cyclic voltammetry (FCV) was applied to quantification of DNA molecules by using a cathodic peak around -1.3 V at a electrode reaction, corresponding to reductive desorption in strong alkali solution. By using a 70.7 mV DC voltage onto a Ag/AgCl reference electrode, the electronic signals of EGFET were applied to detection of DNA molecules and its hybridization, and the corresponding hybridization efficient was estimated to be about 37.5%. About 1 ~ 4 DNA molecules per 100 nm² on the Au substrate of EGFET could be counted, showing a promising sensing technique for bio-molecule.