For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Effect of Different Maceration Levels on the Antioxidant and Bioavailability of Wine from Vitis vinifera Cv. Muscat Hamburg
p.2529
Optimization of Brewing Process of Low-Alcohol Litchi Wine and its Influence on Volatile Compounds
p.2532
Study on the Leaching of Sodium Nitrate from Nitratine
p.2539
Antimicrobial Activities of Extracts of Apocynum venetum Fibers
p.2544
Latrunculin a Enhances the Parthenogenetic Developmental Potentials of Electrically Activated Porcine Oocytes Matured In Vitro
p.2548
Recovery Leaching of Niobium from Metallurgical Residues
p.2554
Effect of Ultrafine Pulverization on Properties of Apple Pomace Powder
p.2560
Antioxidant Activities of the Pyroligneous Acid in Living Caenorhabditis Elegans
p.2564
Synthesis and Potent Biological Activity of 1-Sulfonyl Substituted Imidazole and Benzo[d]imidazole Compounds
p.2570

Latrunculin a Enhances the Parthenogenetic Developmental Potentials of Electrically Activated Porcine Oocytes Matured In Vitro

Article Preview

Abstract:

The present study was carried out to determine the effect of latrunculin A (Lat-A) and FBS on the developmental potentials of electrically activated porcine oocytes matured in vitro. Electrically activated MII oocytes were incubated for 2 hr in mPZM-3 supplemented either with 0、20 nM、1 μM Lat-A or 2.2 μg/ml cytochalasin B (CB). They were then removed and placed in mPZM-3 with or without 10% FBS (addition) on Day 4 for further development. The nuclear activation rate was significantly improved when activated oocytes were cultured in mPZM-3 with Lat-A (P<0.05). At higher dosage (1μM) of Lat-A, the extrusion of second polar body was inhibited while the cleavage rate increased significantly (P<0.05). Significant interaction between Lat-A and FBS supplementation on Day 4 was observed during in vitro development (P<0.05). The hatched blastocyst rates and cell numbers per blastocyst were significantly enhanced with the supplementation of FBS (P<0.05). The blastocyst formation, hatched blastocyst rates and mean cell numbers per blastocyst of 1 μM Lat-A treatment with FBS supplementation were significantly higher than those of 20 nM Lat-A and CB treatments with FBS supplementation (P<0.05), but the individual effect of FBS supplementation was not observed on blastocyst formation in 20 nM and 1 μM Lat-A treatments. While significant difference was seen in blastocyst formation and cell numbers per blastocyst among all the treatments without FBS supplementation (P<0.05). These results indicate that Lat-A especially at the dosage of 1 μM can significantly improve the developmental potential of electrically activated porcine oocytes, and the FBS supplementation on Day 4 of development have an additional effect on porcine embryo development.

You might also be interested in these eBooks
Application of Chemical Engineering View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 236-238)

Pages:

2548-2553

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.236-238.2548

Citation:

Cite this paper

Online since:

May 2011

Authors:

Jian Hui Wang, Dong Min Liu, Yong Le Liu

Keywords:

Activation, Blastocyst, Electrical Pulse, FBS, latrunculin A, Pig Oocyte

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2011 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] M.J. Lohka, M.K. Hayes and J.L. Maller: Proc. Natl. Acad. Sci. USA Vol.85 (1998), p.3009

Google Scholar

[2] M. Whitaker: Physiol. Rev. Vol. 86(2006), p.25

Google Scholar

[3] T. Lorca, F.H. Cruzalegui, D. Fesquet, J.C. Cavadore, J. Mery, A. Means and M. Doree: Nature Vol.366(1993), p.270

Google Scholar

[4] R. Yanagimachi: Mammalian fertilization. In: The Physiology of Reproduction, edited by Knobil E and Neill JD/New York: Raven Press (1994), p.189

Google Scholar

[5] L. Liu, J.C. Ju and X. Yang: Mol. Reprod. Dev. Vol. 49(1998): p.298

Google Scholar

[6] G.S. Lee, H.S. Kim, S.H. Hyun, S.H. Lee, H.Y. Jeon, D.H. Nam, Y.W. Jeong, S. Kim, J.H. Kim, J.Y. Han, C. Ahn, S.K. Kang, B.C. Lee and W.S. Hwang: Theriogenology Vol.63(2005), p.973

DOI: 10.1016/j.theriogenology.2004.04.017

Google Scholar

[7] P. Coy, R. Romar: Reprod. Fertil.Dev. Vol. 14(2002): p.275

Google Scholar

[8] A. Probst, A. Wolken and D. Rath. Theriogenology Vol.57(2002), p.752

Google Scholar

[9] M. Nakai, N. Kashiwazaski, A. Takizawa, Y. Hayashi, E. Nakatsukasa, D.I. Fuchimoto, J. Noguchi, H. Kaneko, M. Shino and K. Kikuchi. Biol. Reprod. Vol. 68(2003), p.1003

DOI: 10.1095/biolreprod.102.009506

Google Scholar

[10] J.L. Tremoleda, T.V. Haeften, T.A.E. Stout, B. Colenbrander and M.M. Bevers. Biol. Reprod. Vol. 69(2003), p.186

Google Scholar

[11] A. Onishi, M. Iwamoto, T. Akita, S. Mikawa, K. Takeda, T. Awata, H. Hanada and A.C.F. Perry. Science Vol. 289(2000), p.1188

DOI: 10.1126/science.289.5482.1188

Google Scholar

[12] W.H. Wang, L.R. Abeydeer, R.S. Prather and N. Billy: Mol. Reprod. Dev. Vol. 51(1998), p.346

Google Scholar

[13] J. Carroll, K.T. Jones and D.G. Whittingham: Rev. Reprod. Vol. 1(1996), p.137

Google Scholar

[14] W.E Houssen, M. Jaspars, K.N. Wease and R.H. Scott. Comp. Biochem. Physiol. C Toxicol. Pharmacol. Vol. 142(2006), p.19

Google Scholar

[15] D. Lim, K. Lange and L. Santella: FASEB J. Vol.16(2002), p.1050

Google Scholar

[16] F.A. Al-Mohanna, E.J. Pettit and M.B. Hallett: Exp. Cell Res. Vol. 234(1997), p.379

Google Scholar

[17] K. Miyoshi, Y. Fujimoto, H. Mori and M. Yoshida: J. Reprod. Dev. Vol. 8(2008), p.159

Google Scholar

[18] F.Z Sun, J. Hoyland, X.Huang, W. Mason and R.M Moor: Development Vol. 115(1992), p.947

Google Scholar

[19] K. Yoshioka, C. Suzuki, A. Tanaka, I.M. Anas and S. Iwamura: Biol. Reprod. Vol. 66(2002), p.112

Google Scholar

[20] L.A. Hyslop, V.L. Nixon, M. Levasseur, F. Chapman, K. Chiba, A. McDougall, J.P .Venables, D.J. Elliott and K.T. Jones: Dev. Biol. Vol. 269(2004), p.206

DOI: 10.1016/j.ydbio.2004.01.030

Google Scholar

[21] L. Lai and R.S Prather: Cloning Stem Cells Vol.5(2003), p.233

Google Scholar

[22] A. Colman: Cloning Vol.1(2000), p.185

Google Scholar

[23] Y.J. Yi and C.S. Park: Anim. Reprod. Sci. Vol.86(2005), p.297

Google Scholar

[24] J.W. Lee, X.C. Tian and X.Z. Yang: Mol. Reprod. Dev. Vol. 68(2004), p.51

Google Scholar

[25] C.L. Leal and L. Liu: Anim. Reprod. Sci. Vol. 52(1998), p.51

Google Scholar

[26] P. Loi, S. Ledda, J. Fulka, P. Cappai and R.M. Moor: Biol. Reprod. Vol.58(1998), p.1177

Google Scholar

[27] T. Schubert and A. Akopian: Neuroscience Vol. 125(2006), p.583

Google Scholar

[28] T. Oka, K. Sato, M. Hori, H. Ozaki and H. Karaki: Br. J. Pharmacol. Vol. 136(2002), p.837

Google Scholar

[29] Z.Y. Zhu, D.Y. Chen, J.S. Li, L. Lian, Z.M. Han, Q.Y. Sun: Biol. Reprod. Vol. 68(2003), p.943

Google Scholar

[30] J.R. Dobrinsky, L.A. Johnson and D.Rath: Biol. Reprod. Vol. 55(1996), p.1069

Google Scholar

[31] B.D. Bavister: Hum. Reprod. Update Vol. 1(1995), p.91

Google Scholar

[32] P. Lonergan, M.O. Kearney-Flynn and M.P. Boland: Theriogenology Vol. 51(1998), p.1565

Google Scholar

[33] C.R. Long, J.R. Dobrinsky and L.A. Johnson: Theriogenology Vol. 51(1998), p.1375

Google Scholar

[34] W.H. Wang and B.N. Day: Zygote Vol.10(2002), p.109

Google Scholar

[35] S. Wang, Y. Liu, G.R. Holyoak and T.D. Bunch: Anim. Reprod. Sci. Vol. 48(1997), p.37

Google Scholar

[36] M.A. Kaaekuahiwi and A.R.J. Menino: J. Anim. Sci. Vol. 68(1990), p.(2009)

Google Scholar

[37] M. Iwamoto, A. Onishi, D. Fuchimoto, T. Somfai, K. Takeda, T. Tagami, H. Hanada, J. Noguchi, H. Kaneko, T. Nagai and K. Kikuchi: Theriogenology Vol.62(2005), p.1585

DOI: 10.1016/j.theriogenology.2004.05.024

Google Scholar

[38] R. De la Fuente and W.A. King: Zygote Vol.5(1997), p.309

Google Scholar

[39] D.L. Davis: J. Reprod. Fertil. Suppl. Vol. 33(1985), p.115

Google Scholar

[40] H.S. Kim, G.S. Lee, S.H. Hyun, S.H. Lee, D.H. Nam, Y.W. Jeong, S. Kim, S.K. Kang, B.C. Lee and W.S. Hwang: Theriogenology Vol.61(2004), p.1381

DOI: 10.1016/j.theriogenology.2003.08.012

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.