Purification of Hulless Barley Anthocyanins with Macroporous Resins
The purification effects of six macroporous adsorption resins on total anthocyanins of hulless barley were compared. The results showed that XAD7HP was the best resin for purification of hulless barley anthocyanins due to its excellent adsorption and desorption capability. The optimal technological parameters were: the pH of sample solution was 3.0, the concentration of sample solution was 21.6 mg/L, the adsorption flow velocity was 1.0 mL/min, the eluting velocity is 1.0 mL/min, and the eluent was about 6.6 bed volumes of 80% ethanol. Reutilization test showed that the XAD7HP resin could be used repeatedly with no significant change of adsorption rate (P>0.05). After purified with XAD7HP, the color value of hulless barley anthocyanins was 41.1, increasing 9.1 times than that of raw extraction.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
T. Zhao et al., "Purification of Hulless Barley Anthocyanins with Macroporous Resins", Advanced Materials Research, Vols. 236-238, pp. 2701-2704, 2011