The Electrochemical Biosensor Based on the Immobilization of Horseradish Peroxidase on TiO2 Nanoneedles Modified Electrodes

Nan Nan Wei; Jiang Yan Du

doi:10.4028/www.scientific.net/AMR.239-242.2499

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 239-242The Electrochemical Biosensor Based on the...

The Electrochemical Biosensor Based on the Immobilization of Horseradish Peroxidase on TiO₂ Nanoneedles Modified Electrodes

Abstract:

A new electrochemical biosensor based on the immobilization of horseradish peroxidase(HRP) on TiO₂ nanoneedles modified electrodes has been fabricated. The direct electrochemical response of HRP immobilized on the modified electrode was dramatically enhanced. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of -0.379 V (vs. SCE). The HRP/TiO₂nanoneedles modified electrode exhibited a remarkable electrocatalytic activity toward the oxidation of H₂O₂. The amperometric response to H₂O₂ showed a linear range of 4–700μmol/L, with the calculated detection limit of 0.78 μmol/L at a signal-to-noise ratio of three. The modified electrode displayed an acceptable reproducibility and good stability. The new HRP/TiO₂nanoneedles matrix is expected to have wide applications for enzymes and proteins immobilization and direct electron transfer study and opened a way for low conductivity electrode biosensors.