Gene Cloning and Bioinformatics Analysis of Glycoprotein H Gene of Pseudorabies Virus Strain SL

Abstract:

The glycoprotein H(gH) gene homologue of pseudorabies virus wild strain SL(PRV-SL) was cloned by degenerate polymerase chain reaction (PCR) from PRV infected vero cells. Bioinformatics analysis was performed to predict the characteristics of gH, the results indicated that gH gene encoded a polypeptide, molecular mass of 71.95kda and comprising 687 amino acids. The protein had one signal peptide between 1 and 24AA, one transmembrane region 645-667AA, 27 antigenic determinants and the hydrophobicity between -2.117 and 3.178. The prediction of secondary structure showed that gH had consisted highly of alpha helix(Hh) and random coil(Cc). The phylogenetic tree showed that PRV-SL was similar to others of the Alphaherpesvirinae, and that gH had higher conservative in Alphaherpesvirinae. Cloning and analysis of gH gene laid foundation to further research and exploitation.

• Periodical: Advanced Materials Research (Volumes 271-273)
• Pages: 1895-1899
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.271-273.1895
• Online since: July 2011
• Authors: Wen Can Jiang, Yang Yang Nian, Ling Zhu, Miao Mei, Zhi Wen Xu, Wan Zhu Guo
• Keywords: Bioinformatic Analysis, gH Gene, Molecular Cloning, PRV SL Strain

References

[1] G. Wittmann, J. Jakubik, and R. Ahl, Multiplication and distribution of Aujeszky's disease (pseudorabies) virus in vaccinated and non-vaccinated pigs after intranasal infection, Archives of Virology, Vol. 66(1980), pp.227-240.

DOI: 10.1007/bf01314736

[2] M. Pensaert and J. Kluge, Pseudorabies virus (Aujeszky's disease), Virus Infections of Porcines, pp.39-64(1989).

[3] J. Moynagh, Aujeszky's disease and the European Community, Veterinary microbiology, Vol. 55(1997), pp.159-166.

[4] T. Fujita, Aujeszky's disease control program in Japan, pp.85-96(1994).

[5] B. Klupp, W. Fuchs, E. Weiland, and T. Mettenleiter, Pseudorabies virus glycoprotein L is necessary for virus infectivity but dispensable for virion localization of glycoprotein H, Journal of virology, Vol(1997). 71, p.7687.

DOI: 10.1128/jvi.71.10.7687-7695.1997

[6] Lisa E. Pomeranz, Ashley E. Reynolds, and Christoph J. Hengartner, Molecular Biology of Pseudorabies Virus: Impact on Neurovirology and Veterinary Medicine, Microbiology and Molecular Biology Reviews, Sept. pp.462-500(2005).

DOI: 10.1128/mmbr.69.3.462-500.2005

[7] L. Pomeranz, A. Reynolds, and C. Hengartner, Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine, Microbiology and Molecular Biology Reviews, Vol. 69(2005), p.462.

DOI: 10.1128/mmbr.69.3.462-500.2005

[8] P. Wu, W. M. Reed, and L. F. Lee. Glycoproteins H and L of Marek's disease virus form a heterooligomer essential for translocation and cell surface expression. Archives of virology Vol. 146(2001), pp.983-992.

DOI: 10.1007/s007050170130

[9] Babic N. , Klupp B. G., Makoschey B, Karger A., Flamand A. and Mettenleiter T. C. Glycoprotein gH of PRV is Essential for Penetration and Propagation in Cell Culture and in the Nervous System of Mice . J Gen V irol , 77(1996), pp.2277-2285.

DOI: 10.1099/0022-1317-77-9-2277

[10] B. Rost, and C. Sander, Prediction of protein secondary structure at better than 70% accuracy, J Mol Biol, Academic Press, England, Vol. 232(1993), pp.584-599.

DOI: 10.1006/jmbi.1993.1413

[11] K. Arnold, L. Bordoli, J. Kopp, and Schwede T. The SWISS-MODEL Workspace: A web-based environment for protein structure homology modelling, Bioinformatics, Oxford University Press , England, Vol. 22(2006), pp.195-201.

DOI: 10.1093/bioinformatics/bti770

[12] YAN Qi-gui, LEI Yan, ZHANG Zhi-he, WANG Cheng-dong, YANG Ai-guo, WANG Xu, ZUO Lan, XIAO Yang. Gene cloning and Bioinformatics Analysis of Outer Capsid Protein VP7 gene for Giant Panda Rotavirus CH-1 Strain. Acta Veterinaria et Zootechnica Sinica, Vol. 41(2010).

DOI: 10.5897/ajb11.1173

[13] Tatiana Gianni, Arianna Cerretani, Rebecca DuBois, Stefano Salvioli, Scott S. Blystone, Felix Rey, and Gabriella Campadelli-Fiume. Herpes Simplex Virus Glycoproteins H/L Bind to Cells Independently of αVβ3 Integrin and Inhibit Virus Entry, and Their Constitutive Expression Restricts Infection. Journal of Virology Vol. 84(2010).

DOI: 10.1128/jvi.02502-09

[14] Huixin Li, Shengwang Liu and Xianggang Kong. Charaterization of the gene encoding UL24, TK and gH proteins from duck enteritis virus (DEV) : a proof for the classification of DEV. Biomedical and Life Sciences, Vol. 33(2006), pp.221-227.

DOI: 10.1007/s11262-005-0060-6

[15] Charles Grose, John E. Carpenter, Wallen Jackson and Karen M. Duus. Overview of Varicella-Zoster Virus Glycoproteins gC, gH and gL. Current Topics in Microbiology and Immunology Vol. 342(2010), pp.113-128.

DOI: 10.1007/82_2009_4