Gene Cloning and Bioinformatics Analysis of Glycoprotein H Gene of Pseudorabies Virus Strain SL
The glycoprotein H(gH) gene homologue of pseudorabies virus wild strain SL(PRV-SL) was cloned by degenerate polymerase chain reaction (PCR) from PRV infected vero cells. Bioinformatics analysis was performed to predict the characteristics of gH, the results indicated that gH gene encoded a polypeptide, molecular mass of 71.95kda and comprising 687 amino acids. The protein had one signal peptide between 1 and 24AA, one transmembrane region 645-667AA, 27 antigenic determinants and the hydrophobicity between -2.117 and 3.178. The prediction of secondary structure showed that gH had consisted highly of alpha helix(Hh) and random coil(Cc). The phylogenetic tree showed that PRV-SL was similar to others of the Alphaherpesvirinae, and that gH had higher conservative in Alphaherpesvirinae. Cloning and analysis of gH gene laid foundation to further research and exploitation.
W. C. Jiang et al., "Gene Cloning and Bioinformatics Analysis of Glycoprotein H Gene of Pseudorabies Virus Strain SL", Advanced Materials Research, Vols. 271-273, pp. 1895-1899, 2011