Study on the Interaction of Fluorescent Probe 2-(2'-Chloro Phenyl)-5-(2'-Hydroxyl Phenyl)1,3,4-Oxadiazole with DNA

Abstract:

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The interaction between self-synthesized fluorescent probe 2-(2'- Chloro phenyl)- 5- (2'- hydroxyl phenyl)1,3,4-oxadiazole (HOXD) and Calf thymus DNA was studied using UV absorption spectra and fluorescence quenching spectra.The data of fluorescence quenching of HOXD by DNA were analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation.The results indicated that the fluorescence quenching of HOXD might be static one attributed to the formation of adduct from HOXD and DNA, which was confirmed by the data of temperature effect experiment. Further results presented in the paper showed that intercalation binding might be the interaction mode between HOXD and DNA.

Info:

Periodical:

Advanced Materials Research (Volumes 284-286)

Main Theme:

Edited by:

Xiaoming Sang, Pengcheng Wang, Liqun Ai, Yungang Li and Jinglong Bu

Pages:

2392-2396

DOI:

10.4028/www.scientific.net/AMR.284-286.2392

Citation:

M. Ding et al., "Study on the Interaction of Fluorescent Probe 2-(2'-Chloro Phenyl)-5-(2'-Hydroxyl Phenyl)1,3,4-Oxadiazole with DNA", Advanced Materials Research, Vols. 284-286, pp. 2392-2396, 2011

Online since:

July 2011

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Price:

$35.00

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