Study on the Interaction of Fluorescent Probe 2-(2'-Chloro Phenyl)-5-(2'-Hydroxyl Phenyl)1,3,4-Oxadiazole with DNA
The interaction between self-synthesized fluorescent probe 2-(2'- Chloro phenyl)- 5- (2'- hydroxyl phenyl)1,3,4-oxadiazole (HOXD) and Calf thymus DNA was studied using UV absorption spectra and fluorescence quenching spectra．The data of fluorescence quenching of HOXD by DNA were analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation．The results indicated that the fluorescence quenching of HOXD might be static one attributed to the formation of adduct from HOXD and DNA, which was confirmed by the data of temperature effect experiment. Further results presented in the paper showed that intercalation binding might be the interaction mode between HOXD and DNA.
Xiaoming Sang, Pengcheng Wang, Liqun Ai, Yungang Li and Jinglong Bu
M. Ding et al., "Study on the Interaction of Fluorescent Probe 2-(2'-Chloro Phenyl)-5-(2'-Hydroxyl Phenyl)1,3,4-Oxadiazole with DNA", Advanced Materials Research, Vols. 284-286, pp. 2392-2396, 2011