Against the problem on poor quality and inefficiency in the preparation of bio tissue micro array by hand, the one processing system of biotissue micro-array was developed. The application of NC image identification technology was made to carry out auto punching, auto-positioning- embedding, and auto-extracting, etc. The auto preparation of biotissue micro array was completed. The adoption of the minimum squares fitting was aimed at object’s primes to be fitted so as to obtain special-feature parameters to finish accurate position and auto rectification of deviation visual view range in micro-hole image. There by, visual deviation operated by hand was avoided and highlighted the processed accuracy.