Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy

Chun Chih Lai; John M. Bell; Nunzio Motta

doi:10.4028/www.scientific.net/AMR.32.49

Paper Titles

The Role of Al₂O₃ Buffer Layer in the Growth of Aligned CNTs
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Improved Fabrication of PMMA/Ag Core-Shell Nanostructures with Two Steps
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Simulation on Optical Properties of Gold and Silver Coated Nano Coreshell
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Effect of Iron Content on the Properties of FePt Nanoparticles in Water in Oil Microemulsions
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Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy
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Prediction of Defects in PZT Thin Film Using Ab-Initio Method
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Nonlinear Analysis of a Ball Grid Array Package under Thermal Cycles
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Deposition and Properties Studies of Superfine TiN Films with Magnetic Filter
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Environmental Response of Plasma Sprayed Nanostructured Coatings
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Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy

Abstract:

A new, direct method has been developed to measure the adhesion forces of bovine serum albumin (BSA) on surfaces by using Atomic Force Microscopy (AFM) in liquid environment. We were able to measure interactions between proteins and substrate surface in PBS solution directly without any modification to the substrate or the AFM tip. Two different surfaces have been used in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The results show that a polystyrene surface is more adhesive to BSA than a mica surface. This is consistent with previous research, which assessed that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique.