Study on Combination and Bioremediation of Chlorimuron-Ethyl-Degrading Strains

Abstract:

The degradation capacity of the four chlorimuron-ethyl-degrading strains, D310-1, LCY-2, LCY-3 and LCY-4, which identified as Rhodococcus sp.,Stenotrophomonas maltophilia sp,Bacillus subtilis sp., and Rhodotorula mucilaginosa sp., respectively, combined in different ways was examined in this study. Then the optimal degradation conditions and mitigation phytotoxicity of chlorimuron-ethyl on sensitive crop were investigated by both orthogonal test and pot culture experiment. The experimental results showed that the combination of four strains was adopted, and the removal rate of chlorimuron-ethyl reached the maximum of 90% in 30 days. Then the optimal degradation conditions were inoculum size 2.5%, temperature 28°C, pH 7.0 and the soil moisture 35%, and the mixed strains could eradicate 92.57% of chlorimuron-ethyl within 30 days. Furthermore pot culture experiment indicated that inoculation of mixed strains could also mitigate the phytotoxic effects of chlorimuron-ethyl on the growth of cucumber seedling.

• Periodical: Advanced Materials Research (Volume 340)
• Pages: 215-221
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.340.215
• Online since: September 2011
• Authors: Chun Hong Xu, Chun Yan Li, Ming Hua Xiong, Jun Bo Pan, Xue Song Li, Xiao Song Cheng
• Keywords: Bioremediation, Chlorimuron-Ethyl-Degrading Strains, Combination, Orthogonal Test

References

[1] H. M. Brown, Mode of action, crop selectivity and soil relations of sulfonylurea herbicides, Pesticide Science, Vol. 29, p.263–281, (1990).

DOI: 10.1002/ps.2780290304

[2] S. S. Pang, C.W. Cuddat, R. G. Dugggleby, Crystallization of Arabidopsis thaliana acetohydroxyacid synthase in complex with the sulfonylurea herbicide chlorimuron-ethyl, , Acta cryst, Vol. 60, pp.153-155, (2004).

DOI: 10.1107/s0907444903025423

[3] I. M. Head, Bioremediation : towards a credible technology, , Microbiology, Vol. 144, pp.599-608, (1998).

DOI: 10.1099/00221287-144-3-599

[4] B. M. Berger, K. Janowitz, H. J. Menne, H. H. Hoppe, Comparative study on microbial and chemical transformation of eleven sulfonylurea herbicides in soil, Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, vol. 105, no. 6, pp.611-623, (1998).

[5] M. Alexander, Biodegradation of chemicals of environmental concern, , Science Vol. 211, no. 4778, p.132–138, January (1981).

[6] X. Pu, T. J. Outright, Degradation of pentachlorophenol by pure and mixed cultures in two different soils, Environmental Science and Pollution Research, Vol. 14, no. 4, pp.244-250. (2007).

DOI: 10.1065/espr2006.07.321

[7] J. Xu, M. Yang, J. Y. Dai, H. Cao, C. P. Pan, X. H. Qiu, M. Q. Xu, Degradation of acetochlor by four microbial communities, Bioresource Technology , vol. 99, no. 16, p.7797–7802, November (2008).

DOI: 10.1016/j.biortech.2008.01.060

[8] J. P. Ma, Z. Wang, P. Lu, H. J. Wang, S. W. Ali, S. P. Li, X. Huang, Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain Pseudomonas sp. LW3, FEMS Microbiol Lett, Vol. 296, p.203–209, (2009).

DOI: 10.1111/j.1574-6968.2009.01638.x

[9] N. Sanyal, S. K. Pramanik, R. Pal, A. Chowdhury, Laboratory simulated dissipation of metsulfuron methyl and chlorimuron ethyl in soils and their residual fate in rice, wheat and soybean at harvest, Journal of Zhejiang University, Vol. 7, no. 3, pp.202-208, (2006).

DOI: 10.1631/jzus.2006.b0202

[10] Y. N. Guo, T. Zhao, Z. Tong, R, S, Tian, Study on combination and ultraviolet mutation of PVA-degrading strains, Chinese Journal of Environmental Engineering, Vol. 4, No. 7, pp.1669-1674, July (2010).

[11] J. L. Chen, K. C. Au, Y. S. Wong, N. F. Yee Tam, Using orthogonal design to determine optimal conditions for biodegradation of phenanthrene in mangrove sediment slurry, Journal of Hazardous Materials, Vol. 176, p.666–671, (2010).

DOI: 10.1016/j.jhazmat.2009.11.083

[12] J.X. Wang, X. H Zhang, W.H. Mu, H. Zhang, Bioremediation of chlorimuron-ethyl contaminated soil by strain 2N3, Chinese Journal of Pesticide Science, Vol. 12, no. 1, pp.49-53, (2010).

[13] M.T. Lee, W.C. Chen, C.C. Chou, Medium improvement by orthogonal array designs for cholesterol oxidase production by Rhodococcus equi, Process Biochem, Vol. 32, no. 8, p.697–703. November (1997).

DOI: 10.1016/s0032-9592(97)00031-9

[14] T.Y. Hsien, Y.H. Lin, Biodegradation of phenolic wastewater in a fixed biofilm reactor, Biochemical Engineering Journal Vol. 27, no. 2, p.95–103, December (2005).

DOI: 10.1016/j.bej.2005.08.023

[15] W. Z. Li, D. S. Fan, Y. B. Luan, Current Situation，problems and solutions of ethametsulfuron，chlorimuron-ethyl and imazethapyr, Chinese Agrochemical, Vo1. 47, no. 11, pp.782-784, November (2008).

[16] R.E. Blackshaw, L.J. Molnar, F.J. Larney, Fertilizer, manure and compost effects on weed growth and competition with winter wheat in western Canada, Crop Protection, Vol. 24, p.971–980, (2005).

DOI: 10.1016/j.cropro.2005.01.021

[17] F. Sun, S. Y. Li, D. J. He, G. Cao, X. Z. Ni, G. H. Tai, et al. Effects of glycoalkaloids from solanum plants on cucumber root growth, Phytochemistry, Vol. 71, p.1534–1538, (2010).

DOI: 10.1016/j.phytochem.2010.06.002