The Effect of Bovine Colostrums IGF-I on Cultured Human Mammary Epithelial Cells Line HBL-100
This experimental studied the effect of the bovine colostrums IGF-I on the cultured human mammary epithelial cells line HBL-100. We selected the best optimal concentration of the bovine colostrums IGF-I on the HBL-100 and researched the cell growth curve, cell population doubling time and cell growth saturation density. Using HPLC to determine lactose content in order to reflect bovine colostrums IGF-I which would influence on the ability of lactation of cultured human mammary epithelial cells line HBL-100. The optimal IGF- I concentration was 200ng/ml, the minimum time of cell population doubling time was 13.8h, cell growth saturation density reached 8.602×105/ml; High concentrations of IGF-Ⅰhad inhibit trend to the HBL-100 cells; bovine colostrums IGF-I could promote the synthesis of the lactose of the HBL-100 significantly.
J. X. Mo et al., "The Effect of Bovine Colostrums IGF-I on Cultured Human Mammary Epithelial Cells Line HBL-100", Advanced Materials Research, Vols. 343-344, pp. 1064-1069, 2012