The Effect of Bovine Colostrums IGF-I on Cultured Human Mammary Epithelial Cells Line HBL-100

Abstract:

This experimental studied the effect of the bovine colostrums IGF-I on the cultured human mammary epithelial cells line HBL-100. We selected the best optimal concentration of the bovine colostrums IGF-I on the HBL-100 and researched the cell growth curve, cell population doubling time and cell growth saturation density. Using HPLC to determine lactose content in order to reflect bovine colostrums IGF-I which would influence on the ability of lactation of cultured human mammary epithelial cells line HBL-100. The optimal IGF- I concentration was 200ng/ml, the minimum time of cell population doubling time was 13.8h, cell growth saturation density reached 8.602×10⁵/ml; High concentrations of IGF-Ⅰhad inhibit trend to the HBL-100 cells; bovine colostrums IGF-I could promote the synthesis of the lactose of the HBL-100 significantly.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 1064-1069
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.1064
• Online since: September 2011
• Authors: Ji Xian Mo, Bo Hui Zhang, Xue Jun Gao
• Keywords: Bovine Colostrums IGF-I, Human Mammary Epithelial Cell, Lactose

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