Effect of Endophytic Fungi on the Growth Situation in Cell Suspension Cultures of Acer Ginnala Maxim

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Abstract:

Fungal endophytes do not harm the plant when they live inside the host. Previous studies also showed that the content of some secondary metabolites are correlated to the endophytes within plant. Whether such a mutualistic symbiosis system could be implanted to bio-engineering or not will be an important and exciting problem. Here a fungal endophyte was co-cultured with the cell suspension of Acer ginnnala which is rich in gallich acid to test the problem above. The results showed that the growth of plant cells and the cell vitality decreased after the endophyte added, while gallic acid content increased. It was preliminarily determined that there is a partial competition relationship.

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Advanced Materials Research (Volumes 343-344)

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384-390

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September 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] G Strobel, et al., Isopesterone anisobenzo-furanone from Pestalotiop sismicrospora, possessing antifungal and antioxidant activities, Phytochemistry, vol. 60, pp.179-183, (2002).

Google Scholar

[2] G A Strobel., Endophytes as sources of bioactive products, Microbes Infect, val. 5, pp.535-554, (2003).

Google Scholar

[3] RX Tan, WX Zou., Endophytes: a rich source of functional metabolites, Natural product reports, val. 18, pp.448-459, (2001).

DOI: 10.1039/b100918o

Google Scholar

[4] JM McDowel, JL Dang. Signal transduction in the plant immune response, TIBS, val. 25, pp.79-82, (2000).

Google Scholar

[5] B Bergman, Johansson C and Soderbac E. The Nostoc-Gunnera symbiosis, New Phytologist, val. 22, pp.379-400, (1992).

DOI: 10.1111/j.1469-8137.1992.tb00067.x

Google Scholar

[6] XM Yu, SX Guo, Establishment of SymbioticSystem for Anoectochilus roxburghii (Wall. ) Lindl. and Endophytic Fungi, China Journal of Chinese Materia Medic. val. 25, no. 2, pp.81-83, (2000).

Google Scholar

[7] K Yoshioka, et al. Induction of apop tosis by gallic acid in human stomach cancer KATO III and colon adenocarcinoma COLO 205 cell lines, Onco. Rep, val. 7, no. 6, pp.1221-1223, (2000).

DOI: 10.3892/or.7.6.1221

Google Scholar

[8] Sakagami H, Satoh K. Prooxidant action of two antioxidant: ascorbic acid and gallic acid J, Anticancer Res, val. 17, no. 1A, pp.221-224, (1997).

Google Scholar

[9] LE Towill,P. Mazur, Studies on the reduction of 2, 3, 5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures , Can.J. Bot, val. 53, pp.1097-1102, (1975).

DOI: 10.1139/b75-129

Google Scholar

[10] CP ZHang, et al. Effects of Fungal Elicitor on Cell Status and Taxol Production in Cells Suspension Cultures of Taxus chinesis var. mairei, Chinese Journal ofBiotechnology, val. 4, no. 17, pp.436-440, (2001).

Google Scholar

[11] Grant JJ, Loake GJ. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance, Plant Physio, val. 24, pp.21-30, (2000).

DOI: 10.1104/pp.124.1.21

Google Scholar

[12] Bradford MM, Anal. Biochem. val. 72, pp.248-252, (1976).

Google Scholar

[13] FH Qi, et al. Fungal endophytes from Acer ginnala Maxim: isolation, identification and their yield of gallic acid, Lettersin App lied Microbiology, val. 49, pp.98-104, (2009).

DOI: 10.1111/j.1472-765x.2009.02626.x

Google Scholar

[14] Avdiushko S.A. et al, Detection of several enzymatic activities in leaf prints cucumber plant, Physiol. Mol. Plant Patho, val. 42, pp.441-454, (1993).

DOI: 10.1006/pmpp.1993.1033

Google Scholar

[15] B E L.D. et al,. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures, Plant Physilo. val. 78, pp.438-441, (1985).

DOI: 10.1104/pp.78.3.438

Google Scholar

[16] MC Brundrett. Coevolution of roots and nycorrhizas of land plants, New Phytologist . val. 15, pp.275-304, (2002).

DOI: 10.1046/j.1469-8137.2002.00397.x

Google Scholar

[17] T. N Sieber., et al, Simultaneous stimulation of endophytic cryptodiapor the hystrix and inhibition of Acer macrophyllum callus in dual culture, Mycologia. val. 82, pp.569-575, (1990).

DOI: 10.2307/3760047

Google Scholar

[18] M Lu et al, Differential growth of Aktinsonella species on host grass calli, Mycologia, val. 86, pp.667-673, (1994).

DOI: 10.1080/00275514.1994.12026467

Google Scholar

[19] J. Hrib, et al, simple callus test to determine the aggressiveness of wood-desrtoying fungi, Eur.J. Forest Pathol. val. 11, pp.270-274, (1981).

DOI: 10.1111/j.1439-0329.1981.tb00096.x

Google Scholar

[20] J Yang, et al, Study on formation of Dragon's blood in Dracaena cochinchinensis inoculated with Fusarium, Chinese Traditional and Herbal Drugs, val. 35, no. 5, p.572 – 574, (2004).

Google Scholar

[21] JW Wang, et al, Elicitation on Artemisinin Biosynthesis in Artemisia annua Hairy Roots by the Oligosaccharide Extract from the Endophytic Colletotrichum sp., Acta Botanica Sinica, val. 44, no. 10, pp.1233-1238, (2002).

Google Scholar